merck millipore,默克密理博,04-1139,Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, rabbit monoclonal

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merck millipore,默克密理博,04-1139,Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, rabbit monoclonal
产品名称：Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, rabbit monoclonal
产品型号：04-1139
Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY is a Rabbit Monoclonal Antibody for detection of phospho-4E-BP1 (Thr70) & has been validated in WB, IHC(P).

merck millipore,默克密理博,04-1139,Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1139,Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1139
描述	Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, rabbit monoclonal
Alternate Names	1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 eIF4E-binding protein 1 eukaryotic translation initiation factor 4E binding protein
背景信息	4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 binds strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.

产品信息
格式	Unpurified
控制	293T cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY is a Rabbit Monoclonal Antibody for detection of phospho-4E-BP1 (Thr70) & has been validated in WB, IHC(P).
主要应用	Western Blotting

生物信息
免疫原品种	Phospho-specific peptide corresponding to residues surrounding Thr70 of human 4E-BP1.
表位	Phosphorylated Thr70
克隆	EPR654(2)AY
宿主	Rabbit
特异性	This antibody detects 4E-BP1 phosophorylated at Thr70.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_004095.3
Entrez基因汇总	This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
基因符号	PHAS-I 4E-BP1 4EBP1 BP-1 MGC4316
修改	Phosphorylation
纯化方法	Unpurified
UniProt编号	Q13541
UniProt汇总	FUNCTION: Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. SUBUNIT STUCTURE: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR. PTM: Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Belongs to the eIF4E-binding protein family.

产品使用声明
质量保证	Evaluated by Western Blot on 293T cell lysates. Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect 4E-BP1 in 293T cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.