merck millipore,默克密理博,04-1040,Anti-Actin Antibody, clone EP184E, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1040,Anti-Actin Antibody, clone EP184E, rabbit monoclonal
产品名称：Anti-Actin Antibody, clone EP184E, rabbit monoclonal
产品型号：04-1040
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Actin Antibody, clone EP184E, Rabbit is an anti More>>

merck millipore,默克密理博,04-1040,Anti-Actin Antibody, clone EP184E, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1040,Anti-Actin Antibody, clone EP184E, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1040
描述	Anti-Actin Antibody, clone EP184E, rabbit monoclonal
Alternate Names	cardiac muscle alpha actin 1 Alpha-cardiac actin actin, alpha, cardiac muscle actin, alpha, cardiac muscle 1
背景信息	Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kDa peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific.

产品信息
格式	Unpurified
控制	A431 cell lysate
演示	Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Actin Antibody, clone EP184E, Rabbit is an antibody against Actin for use in WB, IH(P), IC, IP, FC.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation: 1:20 dilution from a previous lot was used on IP. Flow Cytometry: 1:20 dilution from a previous lot was used on FC. Immunohistochemistry Analysis: 1:100 - 200 dilution from a previous lot detected Actin in human cardiac muscle tissue. Immunocytochemistry Analysis: 1:250 - 500 dilution from a previous lot detected Actin in HeLa cell lysate.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human Actin.
表位	N-terminus
克隆	EP184E
宿主	Rabbit
特异性	This actin antibody recognizes all muscle actin.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	P68032
基因符号	CMH11 ACTC CMD1R
纯化方法	Unpurified
UniProt编号	P68032
UniProt汇总	FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SUBUNIT STRUCTURE: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. INVOLVEMENT IN DISEASE: Defects in ACTC1 are the cause of cardiomyopathy dilated type 1R (CMD1R) [MIM:102540]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in ACTC1 are the cause of cardiomyopathy familial hypertrophic type 11 (CMH11) [MIM:612098]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. SEQUENCE SIMILARITIES: Belongs to the actin family.

产品使用声明
质量保证	Evaluated by Western Blot on A431 cell lysate. Western Blot Analysis: 1:1000 dilution of this antibody was used to detect Actin in A431 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.