merck millipore,默克密理博,17-656,ChIPAb+ Sox-2, clone 6F1.2

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merck millipore,默克密理博,17-656,ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
产品型号：17-656
This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-656,ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-656,ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
M, H	ChIP, WB, ICC

描述
产品目录编号	17-656
商名	Upstate ChIPAb+
描述	ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
Alternate Names	SOX2 Transcription factor SOX-2
背景信息	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin. Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.

产品信息
部件	ChIP Primers, nanog promoter, Part No. CS204374. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human nanog. Store at -20°C. FOR: GTT CTG TTG CTC GGT TTT CT REV: TCC CGT CTA CCA GTC TCA CC Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG), Part No. CS204373. One vial containing 100 μg of protein A purified antibody in 140 μL of 0.02 M phosphate buffer pH 7.6 with 0.25 M NaCl containing 0.1% sodium azide, and 30% glycerol. Store at -20°C. Normal Mouse IgG, Part No. CS200621. One vial containing 125 μg of purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
格式	Purified
控制	Includes negative control mouse IgG antibody and primers specific for human nanog promoter region.

应用
应用	This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Please refer to the EZ-Magna G ChIP™ (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.

生物信息
免疫原品种	Recombinant GST Fusion protein.
克隆	6F1.2
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes Sox-2, MW: ~34 kDa.
同种型	IgG2bκ
品种反应性	MouseHuman
Species Reactivity Note	Human, mouse. Not tested in other species.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003097
Entrez基因汇总	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
基因符号	MCOPS3 SOX2 ANOP3 MGC2413
UniProt编号	P48431
UniProt汇总	FUNCTION: SwissProt: P48431 # Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity). SIZE: 317 amino acids; 34310 Da SUBCELLULAR LOCATION: Nucleus. DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations. SIMILARITY: SwissProt: P48431 ## Contains 1 HMG box DNA-binding domain.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from Ntera 2 cells (3 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1). Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera 2 cells (3 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1).
Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.