merck millipore,默克密理博,FCIM025154,FlowCellect™ Mouse Breg Identification Kit

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merck millipore,默克密理博,FCIM025154,FlowCellect™ Mouse Breg Identification Kit
产品名称：FlowCellect™ Mouse Breg Identification Kit
产品型号：FCIM025154
This FlowCellect Mouse Breg Identification Kit for flow cytometry includes: Anti-Mouse CD5-APC, Anti-Mouse CD19-FITC & Anti-Mouse CD1d-PE, along with optimized assay buffers & a blocking antibody to p More>>

merck millipore,默克密理博,FCIM025154,FlowCellect™ Mouse Breg Identification Kit

产品介绍

merck millipore,默克密理博,FCIM025154,FlowCellect™ Mouse Breg Identification Kit

描述
产品目录编号	FCIM025154
商名	FlowCellect
描述	FlowCellect™ Mouse Breg Identification Kit
概述	Millipore’s FlowCellect™ Mouse Breg Identification Kit includes three directly conjugated antibodies: Anti-Mouse CD5-APC, Anti-Mouse CD19-FITC, and Anti-Mouse CD1d-PE, along with optimized assay buffers and a blocking antibody to provide researchers the ability to phenotypically distinguish cell types. Antibodies against CD5 and CD19 are provided in the kit to phenotype cells by their surface marker expression. CD5 is used to identify B-cell subsets, while CD19 is a mature B-cell marker. CD1d is used to phenotypically identify mouse regulatory B cells, which can serve useful utility in identifying the presence of this B cell subset during certain disease models. Purified Anti-CD16/32 is included for blocking non-specific binding of immunoglobulin to the Fc receptors. All FlowCellect kits are optimized on guava® bench top flow cytometers. FlowCellect kits can be used on any flow cytometer following the same protocol providing researchers a reliable and fully validated solution to study and identify regulatory B cells right in the comfort of their own lab. All three antibodies provided in the kit are carefully titrated and optimized together to ensure maximal performance when run in multiplex, alleviating the need for any additional optimization.
Alternate Names	Flow Cellect
背景信息	Regulatory B cells are a unique subset of small white blood cells that are recognized as a critical component in the immune system. Also known as “Bregs”, the primary function of regulatory B cells are to prevent or dampen overzealous immune responses. This regulatory function appears to be directly mediated by the production of IL-10 (interleukin-10), a potent cytokine which allows the ability of B cells to interact with pathogenic T cells to inhibit harmful immune responses. Bregs have been identified in numerous experimental models such as inflammation, autoimmunity, and cancer, indicating that Breg-mediated suppression is important for maintaining peripheral tolerance. IL-10 production by B cells may suppress the B cell and T cell-mediated immune responses and inflammation that are necessary to efficiently clear pathogens. Understanding Bregs relevance to autoimmune diseases and cancer can assist in the development of novel therapeutic strategies.

产品信息
部件	7. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL. 1. 20X Anti-Mouse CD5-APC, clone 53-7.3: (Part No. CS206436) One vial containing 150 µL. 2. 20X Anti-Mouse CD19-FITC, clone 1D3: (Part No. CS206434) One vial containing 150 µL antibody. 3. 20X Anti-Mouse CD1d-PE, clone 1B1: (Part No. CS206435) One vial containing 150 µL antibody. 4. 20X Anti-Mouse CD16/CD32 Purified, clone 93: (Part No. CS206424) One vial containing 150 µL antibody. 5. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL. 6 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
检测方法	Fluorescent

产品信息

部件

7. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL.
1. 20X Anti-Mouse CD5-APC, clone 53-7.3: (Part No. CS206436) One vial containing 150 µL.
2. 20X Anti-Mouse CD19-FITC, clone 1D3: (Part No. CS206434) One vial containing 150 µL antibody.
3. 20X Anti-Mouse CD1d-PE, clone 1B1: (Part No. CS206435) One vial containing 150 µL antibody.
4. 20X Anti-Mouse CD16/CD32 Purified, clone 93: (Part No. CS206424) One vial containing 150 µL antibody.
5. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
6 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.

检测方法

Fluorescent

应用
应用	This FlowCellect Mouse Breg Identification Kit for flow cytometry includes: Anti-Mouse CD5-APC, Anti-Mouse CD19-FITC & Anti-Mouse CD1d-PE, along with optimized assay buffers & a blocking antibody to phenotypically distinguish cell types.
主要应用	Flow Cytometry

生物信息
宿主	Mixed Species
品种反应性	Mouse
抗体类型	Monoclonal Antibody

产品使用声明
质量保证	Lot to lot in-process testing on all kit components
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.