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merck millipore,默克密理博,FCIM025123,FlowCellect™ Mouse Th1 Intracellular Cytokine Kit
产品名称：FlowCellect™ Mouse Th1 Intracellular Cytokine Kit
产品型号：FCIM025123
This FlowCellect Mouse Th1 Identification Kit for flow cytometry detects IFN-γ expression in mouse Th1 CD4+ T-cells.
merck millipore,默克密理博,FCIM025123,FlowCellect™ Mouse Th1 Intracellular Cytokine Kit
- 产品介绍
描述 产品目录编号 FCIM025123 商名 - FlowCellect
描述 FlowCellect™ Mouse Th1 Intracellular Cytokine Kit 概述 Millipore’s FlowCellect™ Mouse Th1 Identification Kit is designed to enable a researcher a quick and easy way to detect IFN-γ expression in mouse Th1 CD4+ T-cells.
Millipore’s FlowCellect™ Mouse Th1 Identification Kit contains a PE conjugated antibody to IFNγ and a PerCP conjugated antibody to CD4 as well as optimized cytokine blocking reagents and buffers to aid in identifying Th1 CD4+ T-cell subsets in ex vivo lymphocyte populations or to monitor Th1 CD4+ T-cell differentiation in culture. In addition, Millipore has added a fixable viability dye that eliminates false positive data resulting from cytokine staining on dead cells that can occur in long term cultures. The Millipore’s FlowCellect™ Mouse Th1 Identification Kit contains sufficient reagents for 25 3-color tests. Detailed assay instructions are included to assist in analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data. This kit is not designed to be used in the analysis of whole spleen cells from SJL mice.Alternate Names - Flow Cellect
背景信息 There are five main subsets of differentiated CD4+ T-cells; Th1, Th2, Th17, TReg and Tfh cells. Each subtype expresses a signature cytokine or transcription factor that directs the immune response and acts to regulate differentiation. Th1 CD4+ T-cells are important for protection against intracellular bacteria, fungi, protozoa and viruses and are involved in some autoimmune responses. They mediate immune responses to intracellular pathogens and respond by producing interferon gamma (IFN-γ). Through secretion of IFN-γ, Th1 CD4+ T-cells activate macrophages, natural killer (NK) cells and CD8+ T-cells. IFN-γ also increases the production of interleukin-12 by dendritic cells and macrophages, and via positive feedback, stimulates the production of IFN-γ, thereby promoting the Th1 profile. IFN-γ also inhibits the production of interleukin-4, an important cytokine associated with the Th2 subtype, and thus it also acts to preserve its own pathway of differentiation. 产品信息 部件 - 8. 1000X Brefeldin A Buffer: (Part No.CS206347) One vial containing 50 µL.
- 1. 20X Anti-CD4 clone GK1.5-PerCP : (Part No. CS205790) 1 vial containing 150 µL.
- 2. 20X Anti-IFNγ, clone XMG1.2-PE: (Part No.CS206353) One vial containing 150 µL antibody.
- 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL buffer
- 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
- 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
- 6. Permeabilization Buffer Concentrate: (Part No.CS206346) One bottle containing 15 mL buffer
- 7. 1000X Fixable Viability Dye eFluor®660: (Part No.CS206348) One vial containing 50 µL.
检测方法 Fluorescent 应用 应用 This FlowCellect Mouse Th1 Identification Kit for flow cytometry detects IFN-γ expression in mouse Th1 CD4+ T-cells. 主要应用 - Flow Cytometry
生物信息 宿主 Mouse 品种反应性 Mouse 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 4-8°C for antibodies and buffers 包装信息 数量 25 Tests / Kit
上一件merck millipore产品：merck millipore,默克密理博,681675,Wortmannin - CAS 19545-26-7 - Calbiochem
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