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  • merck millipore,默克密理博,FCCH100138,FlowCellect™ Human T Cell Apoptosis Kit

  • merck millipore,默克密理博,FCCH100138,FlowCellect™ Human T Cell Apoptosis Kit

    产品名称：FlowCellect™ Human T Cell Apoptosis Kit
    产品型号：FCCH100138
    The FlowCellect T cell Apoptosis Kit allows for the detection of T-lymphocytes & T-lymphocyte subsets in peripheral blood mononuclear cell samples & provides information on the apoptotic status of the More>>

    merck millipore,默克密理博,FCCH100138,FlowCellect™ Human T Cell Apoptosis Kit

  • 产品介绍
  • merck millipore,默克密理博,FCCH100138,FlowCellect™ Human T Cell Apoptosis Kit

    描述
    产品目录编号 FCCH100138
    商名
    • FlowCellect
    描述 FlowCellect™ Human T Cell Apoptosis Kit
    概述 The FlowCellect T cell Apoptosis Kit allows for the detection of T-lymphocytes and T-lymphocyte subsets in peripheral blood mononuclear cell samples and simultaneously provides information on the apoptotic status of these cells as measured by Annexin V binding, all in a simple easy to use assay utilizing flow cytometry. The kit thus allows the determination of % of T cells and the subset of apoptotic T cells which are apoptotic/necrotic. When performed on the guava easyCyte 8HT system, it can determine the count of population without using external bead sets. Multiparametric evaluation of T Cell subpopulations along apoptotic status is of great utility in studying impact of compounds on immune cell subpopulations in drug screening studies; in the study of immuno-modulatory drugs, and in immunotoxicity studies. It can provide an understanding of the mechanistic machinery of both normal and abnormal immune cell apoptosis; in addition it can provide insights into mechanism of disease and immune dysfunction and can also be of great value in quality control of immune cell sub-populations. The kit provides a complete picture of T cell apoptotic status and its response for inducer treatment conditions or diseases. Sufficient reagents are provided for 100 tests.
    Alternate Names
    • Flow Cellect
    背景信息 Apoptosis plays a critical role in immune cell development, regulation and functioning of the immune system and is also a contributor to the pathology of many human diseases and disorders of immune function. Apoptosis provides the mechanism for deletion of autoreactive T cells in the thymus, low responsive B cells in the germinal center, and of target cells attacked by cytotoxic T lymphocytes and natural killer cells. The process of apoptosis is accompanied by multiple morphological, biochemical and physiological changes in response to specific induction signals. Among these are externalization of phosphatidylserine (PS) to the cell surface, cleavage and degradation of specific cellular proteins, compaction and fragmentation of nuclear chromatin, and loss of membrane integrity.
    产品信息
    部件
    • 4. 10X Assay Buffer HSC (Part No. 4700-1325) One bottle containing 10mL of buffer.
    • 1. CD8-FITC/CD4-PE/CD3-PECy5 (Part No.4700-1355) One vial containing 1000 µL of cocktail.
    • 2. Annexin V, CF647 Reagent (Part No. 4300-0325) One vial containing 500 µL Annexin V, CF647.
    • 3. 1X Assay Buffer BA: (Part No. 4700-1360) One bottle containing 50 mL of buffer.
    检测方法 Fluorescent
    应用
    应用 The FlowCellect T cell Apoptosis Kit allows for the detection of T-lymphocytes & T-lymphocyte subsets in peripheral blood mononuclear cell samples & provides information on the apoptotic status of these cells as measured by Annexin V binding.
    主要应用
    • Flow Cytometry
    生物信息
    宿主 Mouse
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    产品使用声明
    质量保证 Lot to lot in-process testing on all kit components
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 4-8°C for antibodies and buffers
    包装信息
    数量 100 Tests / Kit

    merck millipore,默克密理博,FCCH100138,FlowCellect™ Human T Cell Apoptosis Kit

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