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重要规格表
主要应用 ELISA 描述 产品目录编号 EPA100 品牌系列 Chemicon®
商名 - Chemicon
描述 EPA Peroxidase Protection Buffer 概述 Peroxidase Protective Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods. 产品信息 演示 Peroxidase Protective Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2. 应用 应用 500 mL EPA Peroxidase Protection Buffer for ELISA. 主要应用 - ELISA
应用说明 USE:
Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in Peroxidase Protective Buffer. Diluted conjugates are stable for at least 3 months when stored at 2 - 8oC. The buffer is suitable for direct use in ELISA. It is recommended that the HRP conjugated antibody is diluted with Peroxidase Protective Buffer and allowed to stand for 24 hours at 2-8°C to attain constant stability before use.
Peroxidase Protective Buffer is compatible with blocking agents such as skim milk powder, Tween 20 and normal serum. However HRP conjugates should not be stored in the presence of these blocking agents. They should be added just prior to use in the assay.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at +2 to +8°C, for up to 6 months.
PRECAUTIONS
HRP conjugates should be diluted and stored under clean conditions to reduce the level of microbial challenge.
Sodium azide must NOT be added to Peroxidase Protective Buffer.
Avoid contamination of HRP conjugate solutions with heavy metal ions, aromatic chlorocarbons or hypochlorous acids. Avoid containers made of soda glass or polystyrene. Storage of diluted conjugates in borosilicate glass is recommended.
Efficient washing steps should follow HRP conjugate incubations. In ELISA, at least four thorough wash cycles are recommended.包装信息 数量 500 mL
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