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merck millipore,默克密理博,DR1025,PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb
产品名称:PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb
产品型号:DR1025
This PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).
merck millipore,默克密理博,DR1025,PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells. 产品目录编号 DR1025 品牌系列 Calbiochem®
应用数据 
Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.参考 参考 Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
Zeng, Y., et al. 1998. Nature 395, 507.产品信息 形式 Liquid 配方 In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. 正控制 Lysates from COS cells treated with UV 防腐剂 None 应用 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human, monkey, mouse, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Do not aliquot.
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