merck millipore,默克密理愽
  • merck millipore,默克密理博,05-1510,Anti-Aly/REF Antibody, clone 11G5

  • merck millipore,默克密理博,05-1510,Anti-Aly/REF Antibody, clone 11G5

    产品名称：Anti-Aly/REF Antibody, clone 11G5
    产品型号：05-1510
    Anti-Aly/REF Antibody, clone 11G5 is a Mouse Monoclonal Antibody for detection of Aly/REF also known as Ally of AML-1 & LEF-1 or THO complex 4 & has been validated in WB, ICC, IP & IHC.

    merck millipore,默克密理博,05-1510,Anti-Aly/REF Antibody, clone 11G5

  • 产品介绍
  • merck millipore,默克密理博,05-1510,Anti-Aly/REF Antibody, clone 11G5

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HIHC, WB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-1510
    描述 Anti-Aly/REF Antibody, clone 11G5
    Alternate Names
    • transcriptional coactivator
    • Ally of AML-1 and LEF-1
    • THO complex 4
    • Transcriptional coactivator Aly/REF
    • bZIP enhancing factor
    • bZIP-enhancing factor BEF
    背景信息 Aly (THO complex subunit 4 or THOC4 or Aly/REF) is a heat stable, nuclear protein that functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. Aly (THOC4) is also preferentially associated with intronless mRNAs in the nucleus. Loading of export factors onto mRNAs is a key step in gene expression. In vertebrates, splicing plays a role in this process. Specific protein complexes, exon junction complexes and transcription/export complexes, are loaded onto mRNAs in a splicing-dependent manner, and adaptor proteins such as Aly (THOC4) in the complexes in turn recruit mRNA exporters onto the RNA.
    产品信息
    格式 Purified
    控制
    • Hek293 cell lysate
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Anti-Aly/REF Antibody, clone 11G5 is a Mouse Monoclonal Antibody for detection of Aly/REF also known as Ally of AML-1 & LEF-1 or THO complex 4 & has been validated in WB, ICC, IP & IHC.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Aly/REF in NIH/3T3 and HeLa cells.

    Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Kim, V.N., et al. (2001). The EMBO Journal. 20, 2062-2068.)
    生物信息
    免疫原品种 Recombinant protein corresponding to human Aly/REF.
    表位 Unknown
    克隆 11G5
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes Aly/REF.
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_005773.3
    Entrez基因汇总 The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq].
    基因符号
    • THOC4
    • ALY
    • BEF
    • Tho4
    纯化方法 Protein G purfied
    UniProt编号
    • Q86V81
    UniProt汇总 FUNCTION: Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway.

    SUBUNIT STRUCTURE: Homomultimer. Binds LEF1 and RUNX1 By similarity. Is part of several complexes involved in mRNA processing and export. Part of the heteromultimeric THO/TREX complex containing THOC1, THOC2, THOC3, THOC4 and UAP56. Associates with the spliceosome. Binds NXF1 and RBM8A directly and is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RBM8A, SRRM1, NXF1, RENT2, RENT3B and THOC4 By similarity. Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and THOC4. Binds UL54 from Herpes simplex type 1 (strain KOS). Found in a mRNP complex with RENT3A and RENT3B. Interacts with BAT1, RBM8A, RNPS1 and SRRM1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.

    SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.

    PTM: Arg-50 and Arg-204 are dimethylated, probably to asymmetric dimethylarginine.

    Phosphorylated upon DNA damage, probably by ATM or ATR.

    INVOLVEMENT IN DISEASE: Antibodies against THOC4 are found in sera of patients with systemic lupus erythematosus (SLE).

    SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
    产品使用声明
    质量保证 Evaluated by Western Blot in Hek293 cell lysate.

    Western Blot Analysis: 0.5 µg/ml of this antibody detected Aly/REF on 10 µg of Hek293 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,05-1510,Anti-Aly/REF Antibody, clone 11G5

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