merck millipore,默克密理博,DR1017,PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb

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Merck Millipore 默克密理愽

宿主
Rb

描述
概述	Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form phosphorylated at Ser¹³⁹.
产品目录编号	DR1017
品牌系列	Calbiochem®
应用数据	Detection of human and mouse γ-H2AX, phosphorylated at Ser¹³⁹ by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, γ-H2AX embryonic fibroblasts (+/+), and mouse γ-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-γ-H2AX (pSer¹³⁹) Rabbit pAb (1:10,000). Detection: chemiluminescence.

参考
参考	Stiff. T., et al. 2004. Cancer Res. 64, 2390. Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273. Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579. Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.

产品信息
形式	Liquid
配方	In Tris/citrate-phosphate buffer, pH 7.0-8.0.
正控制	HEK293 cell nuclear extract
防腐剂	≤0.1% sodium azide

应用
主要应用	Immunoblotting (Western Blotting) Immunocytochemistry

生物信息
免疫原	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹³⁹ phosphorylation site of human H2AX
宿主	Rabbit
同种型	IgG
品种	human, mouse
浓缩标签	Please refer to vial label for lot-specific concentration

储存和货运信息
货运代码	Blue Ice Only
毒性	Highly Toxic
储存	+2°C to +8°C
无需冷冻	No
特殊指南	Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

merck millipore,默克密理博,DR1017,PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb

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