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merck millipore,默克密理博,CYT298,C-Reactive Protein ELISA Kit, Human
产品名称:C-Reactive Protein ELISA Kit, Human
产品型号:CYT298
The human C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures human CRP.
merck millipore,默克密理博,CYT298,C-Reactive Protein ELISA Kit, Human
- 产品介绍
重要规格表
可用的分析物 品种反应性 主要应用 C-Reactive Protein H ELISA 描述 产品目录编号 CYT298 品牌系列 Chemicon®
商名 - Chemicon
描述 C-Reactive Protein ELISA Kit, Human 概述 Introduction
The human C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures human CRP.
Test Principle
With the human CRP ELISA, standards, quality controls and samples of sera are incubated in microtitration wells coated with polyclonal anti-human CRP antibody. After a thorough wash, polyclonal anti-human CRP antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-CRP complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of CRP. A standard curve is constructed by plotting absorbance values versus CRP concentrations of standards, and concentrations of unknown samples are determined using this standard curve.Alternate Names - CRP
产品信息 部件 - Wash Buffer, 1 packet (PBS with 0.05% Tween 20, pH 7.4 when reconstituted to 1 liter of distilled water) (Catalog Number CYT298f)
- Microwell Strips (96 wells), coated with Anti-human CRP Antibody, vacuum sealed (Catalog No. CYT298a)
- Conjugate Concentrate (100x) (Anti-human CRP Antibody, Horseradish Peroxidase Conjugate), 0.13 mL (Catalog No. CYT298b)
- Human CRP Standard, 1.0 mg/mL (100x), 0.13 mL, (Catalog No. CYT298c)
- Substrate (TMB) Solution, 12 mL, Protect from light. Non-carcinogenic. (Catalog No. CYT298d)
- Stop Solution (diluted phosphoric acid), 12 mL (Catalog No. CYT298e)
应用 应用 The human C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures human CRP. 主要应用 - ELISA
应用说明 The human C-reactive protein (CRP) kit is designed to measure the amount of human CRP in serum or plasma. There are enough reagents included in this kit for one 96-well immuno-assay plate. We recommend running duplicate wells for samples and standards.
For Research Use Only; Not for use in diagnostic procedures.生物信息 品种反应性 Human 可用的分析物 - C-Reactive Protein
Entrez基因编号 - NM_000567.2
基因符号 - MGC149895
- CRP
- PTX1
- MGC88244
UniProt编号 - P02741
UniProt汇总 FUNCTION: SwissProt: P02741 # Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
COFACTOR: Binds 2 calcium ions per subunit.
SIZE: 224 amino acids; 25039 Da
SUBUNIT: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Found in plasma.
SIMILARITY: SwissProt: P02741 ## Belongs to the pentaxin family. & Contains 1 pentaxin domain.
MISCELLANEOUS: This protein owes its name to its ability precipitate pneumococcal C-polysaccharide in the presence of calcium.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store the kit at 2° to 8°C for up to 6 months after date of receipt or until expiration date.
Precautions
· This kit contains components of human origin. These materials were found non-reactive for hepatitis B surface antigen and for HIV antibody. However, these materials should be handled as potentially infectious, as no tests can guarantee the complete absence of infectious agents.
· Avoid contact with the acidic Stop Solution and Substrate Solution, which contains urea peroxide. Wear gloves and eye protection when handling these reagents. In case of contact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and seek medical attention, when necessary.
· HRP-labeled conjugate and TMB-substrate are photosensitive and are packaged in a protective opaque bottle. Store in the dark and return to storage after use.
· Wear gloves and laboratory coats when handling the kit.
· The materials must not be pipetted by mouth.
· Do not drink, eat or smoke in the areas where the kit is being handled.
· Reagents with different lot numbers should not be mixed.包装信息 数量 1 plate 包装 96 wells
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