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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 CBL147 Replaces CBL147PC 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-BL-CAM Antibody, clone RFB4 Alternate Names - CD22
产品信息 格式 Purified 演示 The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. 应用 应用 Anti-BL-CAM Antibody, clone RFB4 detects level of BL-CAM & has been published & validated for use in FC, IF, IP, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Identification of B lymphocytes and HCL cells by indirect immunofluorescence staining
Identification of B lymphocytes and B lymphocyte progenitor cells by staining of cytoplasmic CD22 antigen using the APAAP technique
Staining of B lymphocytes in frozen tissue sections by indirect immunoperoxidase staining
Immunoprecipitation of CD22 antigen
Signal transduction studies of CD22 which is phosphorylated following slgM cross-linking
Studies on the interaction of CD22 and its ligand, CD45
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Human tonsil lymphocytes 克隆 RFB4 宿主 Mouse 特异性 The CD22 antigen is a heterodimer composed of two glycoprotein chains of 130-140 kDa and is present as both a cytoplasmic and membrane antigen on B lymphocytes. Antigen expression appears at an early stage in B lymphocyte differentiation at approximately the same stage as the CD19 antigen. Surface expression of the CD22 antigen by B lymphocytes is variable. Circulating B lymphocytes are CD22 positive and follicular mantle and marginal zone B lymphocytes show strong binding of this antibody. Germinal centre B cells exhibit only weak expression of the CD22 antigen. The CD22 antigen is poorly expressed by non T ALL cells and by a proportion of B CLL cells. Hairy cell leukaemia cells have been demonstrated to strongly express the CD22 antigen. CD22 functions as a sialic acid binding lectin specifically sialic acid in alpha-2,6 linkage. Sialylation of CD22 by alpha-2,6 sialyl transferase inhibits CD22 ligand binding.
Antigen distribution:
B cells (lg+, E-) 92 + 3%
Peripheral blood lymphocytes 12 + 3%
Thymocytes < 1%
Granulocytes < 1%
Monocytes > 1%
T cells (E+) > 1%
FUSION PARTNER: NS1 myeloma cell line同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001771.2
基因符号 - SIGLEC2
- CD22
- Siglec-2
- SIGLEC-2
- BL-CAM
- FLJ22814
- MGC130020
- Leu-14
UniProt编号 - P20273
UniProt汇总 FUNCTION: SwissProt: P20273 # Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
SIZE: 847 amino acids; 95348 Da
SUBUNIT: Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: B-lymphocytes.
DOMAIN: SwissProt: P20273 Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2- containing phosphatases.
PTM: Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. & Phosphorylated on tyrosine residues by LYN (By similarity).
SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. & Contains 6 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,AB1592P,Anti-NMDAR2C Antibody, also recognizes NMDAR2A and 2B
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