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merck millipore,默克密理博,CBL1358,Anti-Endoglin Antibody, Extracellular, clone MJ7/18
产品名称：Anti-Endoglin Antibody, Extracellular, clone MJ7/18
产品型号：CBL1358
This Anti-Endoglin Antibody, Extracellular, clone MJ7/18 is validated for use in FC, IP, WB, IH for the detection of Endoglin.
merck millipore,默克密理博,CBL1358,Anti-Endoglin Antibody, Extracellular, clone MJ7/18
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M IHC, FC, WB R Purified Monoclonal Antibody 描述 产品目录编号 CBL1358 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Endoglin Antibody, Extracellular, clone MJ7/18 Alternate Names - Ancillary TGF-beta Receptor
- CD105
背景信息 Endoglin is the regulatory component of the TGF-beta receptor complex. CD105 is a homodimer of 90 kDa subunits and is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition, stromal cells in the connective tissue of various organs are also positive for endoglin expression. CD105 has been reported to have functions in adhesion and embryonic angiogenesis, and that MJ7/18 is a pan endothelial marker (with minimal reactivity with early hematopoetic cells). Both mouse and human CD105 exhibit >70% sequence similarity in their cytoplasmic domains with type III transforming growth factor β (TGFβ) receptor. 产品信息 格式 Purified 控制 - POSITIVE CONTROL: mouse vascular endothelial cells
演示 Protein A purified immunoglobulin presented as a liquid in 100 mM borate buffered saline, containing no preservatives. 应用 应用 This Anti-Endoglin Antibody, Extracellular, clone MJ7/18 is validated for use in FC, IP, WB, IH for the detection of Endoglin. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
不建议的应用 - Immunohistochemistry (Paraffin)
应用说明 Flow Cytometry: 1μg/ 10(e6) cells in 100 μL total volume.
Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.
Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.
Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Inflamed mouse skin (Ge, AZ et al, 1994). 克隆 MJ7/18 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 Specifically recognizes mouse CD105, known as endoglin. 同种型 IgG2aκ 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000118.1
Entrez基因汇总 Endoglin is a homodimeric transmembrane glycoprotein highly expressed by endothelial cells. It is a component of the transforming growth factor beta receptor complex as it binds TGFB1 and TGFB3 with high affinity. Mutations in the endoglin gene produce hereditary hemorrhagic telangiectasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. 基因符号 - endoglin
- ENG
- ORW
- CD105
- HHT1
- END
- FLJ41744
- ORW1
UniProt编号 - P17813
UniProt汇总 FUNCTION: SwissProt: P17813 # Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
SIZE: 658 amino acids; 70578 Da
SUBUNIT: Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGF-beta receptors I and/or II. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Endoglin is restricted to endothelial cells in all tissues except bone marrow.
DISEASE: SwissProt: P17813 # Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 12 months from date of receipt. 包装信息 数量 500 µg
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下一件merck millipore产品：merck millipore,默克密理博,362225,β₂-Glycoprotein 1, Human Plasma
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