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描述 概述 A sensitive and robust homogeneous PI 3-K assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter. 产品目录编号 CBA101 品牌系列 Calbiochem®
同义词 Phosphoinositide 3 Kinase Assay Kit 应用数据 
Varying concentrations of enzyme were mixed with 1 µM substrate in cAB containing 75 µM ATP. Relative fluorescence units (RFU) decrease as a function of enzyme activity.
Varying amounts of ATP were used in the absence or presence of enzyme. Percent activity was calculated based on the ΔRFU between the samples.
Varying amounts of Wortmannin in 0.05% DMSO were added to enzyme (32 nM) and substrate (1 µM) in cAB containing 20 µM ATP.
Statistical data were produced using 0% (top) and 100% phospho-calibrators (bottom) or 27 nM PI 3-Kα (middle). Z′-factors of 0.8 and 0.7 were obtained using phospho-calibrators or reacted samples, respectively. A Z′-factor of >0.5 indicates a robust assay.参考 参考 Ruckle, T., et al. 2006. Nat. Rev. Drug Discov. 11, 903.
Zhao, J.J., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 16296.
Zhao, J.J. and T.M Roberts. 2006. Sci STKE 365, 52.
Zhao, J.J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 18443.
Meier T.I., et al. 2004. Protein Expr. Purif. 35.218.产品信息 形式 100 Tests 格式 96-well plate 试剂盒包含 Assay Buffer, Coordination Buffer, Sensor Stock, Sensor Dilution Buffer, Trivalent Metal Ion, Post Reaction Buffer, and a user protocol. 生物信息 化验时间 2 h 样品类型 Purified enzyme 安全信息 R相 R: 10-23/24/25-39/23/24/25
Flammable.
Toxic by inhalation, in contact with skin and if swallowed.
Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed.S相 S: 1/2-36/37-45-63-A09
Keep locked up and out of the reach of children.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
In case of accident by inhalation: remove casualty to fresh air and keep at rest.
产品使用声明 预定用途 The Calbiochem® PI 3-K Assay Kit is a homogeneous assay designed to measure PI 3-K activity using purified enzyme preparations. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 +2°C to +8°C
存储条件 Upon arrival store the entire contents of the kit at 4°C. 无需冷冻 No
补充信息 试剂盒包含 Assay Buffer, Coordination Buffer, Sensor Stock, Sensor Dilution Buffer, Trivalent Metal Ion, Post Reaction Buffer, and a user protocol.
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