merck millipore,默克密理博,14-781,PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg

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Merck Millipore 默克密理愽

描述
产品目录编号	14-781
商名	Upstate
描述	PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg
概述	Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation E545K and untagged, recombinant, full length mouse p85α.
背景信息	The E545K substitution is associated with a number of human cancers. This residue is conserved between the human and mouse sequences of p110α. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.

应用
应用	Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation E545K & untagged, recombinant, full length mouse p85α. For use in Kinase Assays.
主要应用	Kinase Assay

生物信息
来源	Coexpressed by baculovirus in Sf21 insect cells.
品种	Mouse
特异活性	For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez基因编号	NM_006218.2
Entrez基因汇总	Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
基因符号	MGC142163 PIK3CA PI3K MGC142161 p110-alpha
蛋白靶	PI3Kα/p85α
纯化方法	Ni²⁺/NTA-agarose
靶子系列	PI3K/PI4K
UniProt编号	P42336
UniProt汇总	FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. SIZE: 1068 amino acids; 124284 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity). DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370]. SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.

储存和货运信息
存储条件	Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

包装信息
数量	10 µg
包装	Also available in 250 μg size --call for pricing and availability and reference catalog number 14-781M when ordering the 250 μg size.

merck millipore,默克密理博,14-781,PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg

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