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重要规格表
检测方法 Fluorescence 描述 概述 Dipeptidyl peptidase IV (DPP IV) is a serine exopeptidase. The natural substrate of this enzyme include chemokines, cytokines, endomorphines, hormones and the pancreatic polypeptide of the PACAP/glucagons peptide family. Of particular interest is the ability of DPP IV to regulate incretin hormones. Since these hormones are deactivated by DPP IV, a multivalent method of treatment for type 2 diabetes and, more generally, in glucose metabolism disorders, is opened by the inhibition of this protease. 产品目录编号 CBA085 品牌系列 Calbiochem®
应用数据 
The activities of recombinant human DPP II, FAP, and DPP I were assessed as outlined in the Detailed Protocol above.
Human recombinant DPP IV activity was measured as outlined in the Detailed Protocol above using a 1-h incubation at 37°C. The data is presented as Relative Fluorescence Units.
Cells (cell types are denoted in the figure) were cultured in DMEM with 10% FCS and harvested at 80-90% confluency. Total cell lysates were prepared using CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) according to the manufacturer's recommended protocol. Human spleen extract was prepared by homogenizing tissue with 170 mM NaCl, 2 mM EDTA. Human plasma (pooled samples) was obtained from commercial sources. The concentration of active DPP IV was determined by extrapolating the RFU values to the standard curve prepared with human recombinant DPP IV. Protein concentration in cell lysates and tissue extract was determined using a BCA protein assay. Activity was assessed as outlined in the Detailed Protocol above. Fluorescence intensity was measured at an excitation wavelength of 360 nm and an emission wavelength of 460 nm and expressed per mg total protein.参考 参考 McIntosh, C.H. 2008. Front. Biosci. 13, 1753.
Thompson, M.A., et al. 2007. Mini Rev Med Chem. 7, 253.
Demuth, H.U., et al. 2005. Biochim Biophys Acta. 1751, 33.
Gallwitz, B. 2005. Rev Diabetic Stud. 2, 61.
Pro, B., and Dang, N.H. 2004. Histol Histopathol. 19, 1345.
Durinx, C., et al. 2000. Eur J Biochem. 267, 5608.产品信息 检测方法 Fluorescence 形式 96 Tests 格式 96-well plate 试剂盒包含 Anti-DPP IV Antibody 96-well Coated Plate, DPP IV Control, DPP IV Substrate, Assay Buffer, 10X Sample Buffer, Plate Sealer, and a user protocol 生物信息 化验范围 0.31-20 ng/ml 品种 human 安全信息 R相 R: 36/37/38
Irritating to eyes, respiratory system and skin.S相 S: 26-37/39-56
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable gloves and eye/face protection.
Dispose of this material and its container at hazardous or special waste collection point.产品使用声明 预定用途 The Calbiochem® InnoZyme™ DPP IV Immunocapture Activity Assay is intended for the measurement of human DPP IV activity in biological samples such as cell lysates, tissue extracts, and biological fluids. 储存和货运信息 货运代码 Dry Ice Only
毒性 Irritant
储存 -20°C
存储条件 Upon arrival store the unopened kit at -20°C. All kit components, once opened, can be stored for up to 3 months under the following conditions: Table 1: Storage Conditions
Note: Following initial use the Control should be dispensed into aliquots and stored at -20°C or -70°C. Avoid freeze/thaw cycles.无需冷冻 No
补充信息 试剂盒包含 Anti-DPP IV Antibody 96-well Coated Plate, DPP IV Control, DPP IV Substrate, Assay Buffer, 10X Sample Buffer, Plate Sealer, and a user protocol
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