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merck millipore,默克密理博,CA1023,Anti-Hsp90α Mouse mAb (EMD-17D7)
产品名称：Anti-Hsp90α Mouse mAb (EMD-17D7)
产品型号：CA1023
This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.
merck millipore,默克密理博,CA1023,Anti-Hsp90α Mouse mAb (EMD-17D7)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β. 产品目录编号 CA1023 品牌系列 Calbiochem®
同义词 Anti-Heat Shock Protein 90α 应用数据 
Detection of human Hsp90α by immunoblotting. Samples: Whole cell lysate (50 μg) from HeLa cells (lane A), Hsp90β, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 μg/ml). Detection: chemiluminescence.
Detection of human Hsp90α by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 μg) from H-460 cells. Antibody for IP: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 μg/700 μg protein). Conditions for immunoblotting: Sample: 10 μl of 50 μl Hsp90α IP (above). Primary antibody: Anti-Hsp90α Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.参考 参考 Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
Nemoto, T., et al. 1998. Biochem. J. 330, 989.产品信息 形式 Liquid 配方 In PBS. 正控制 H460 and HeLa cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) 免疫原品种 Human 克隆 EMD-17D7 宿主 Mouse 同种型 IgG₁ 品种 human 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Blue Ice Only
毒性 Irritant
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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