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merck millipore,默克密理博,MABN685,Anti-KDM4A Antibody, clone5H1
产品名称：Anti-KDM4A Antibody, clone5H1
产品型号：MABN685
Anti-KDM4A Antibody, clone5H1 is a highly specific mouse monoclonal antibody, that targets Jumonji domain-containing protein (JMJD) & has been tested in western blotting, IHC & Immunofluorescence.
merck millipore,默克密理博,MABN685,Anti-KDM4A Antibody, clone5H1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MABN685 描述 Anti-KDM4A Antibody, clone5H1 Alternate Names - KDM4A
- Lysine-specific demethylase 4A
- JmjC domain-containing histone demethylation protein 3A
- Jumonji domain-containing protein 2A
背景信息 Lysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle or developmental stage. KDM4A demethylates H3K36me3, a modification enriched in the 3′ end of active genes. KDM4A is also known as Lysine-specific demethylase 4A or JmjC domain-containing histone demethylation protein 3A, or Jumonji domain-containing protein 2A, and is encoded by the human gene named KDM4A/JHDM3A/JMJD2/JMJD2A/KIAA0677. KDM4A is the histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone gene repression, and KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated forms. KDM4A does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20', but KDM4A does demethylate trimethylated H3 'Lys-9' and H3 'Lys-36' residues, while it has no activity on mono- and dimethylated residues. KDM4A also plays a critical role in muscle differentiation as demethylation of various targets allows influences the expression of the Myog gene family critical in muscle cell development. KDMA4A also interacts with histone deacetylases HDAC1, HDAC2, and HCAC3. It is localized in the nucleus and widely expressed. KDM4A’s own levels are mediated via ubiquitination and proteasome degradation. EMD-Millipore’s Anti-KDM4A mouse monoclonal antibody has been tested in western blot on HEK293 and KDM4A transfected HEK293 cell lysates and in paraffin embedded immunohistochemistry on human colon and larynx cancer tissues and in fluorescent immunocytochemistry on NTERA-2 cells in culture. 产品信息 格式 Ascites 控制 - HEK293 and KDM4A-hIgGFc transfected HEK293 cell lysates
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Anti-KDM4A Antibody, clone5H1 is a highly specific mouse monoclonal antibody, that targets Jumonji domain-containing protein (JMJD) & has been tested in western blotting, IHC & Immunofluorescence. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KDM4A in human colon cancer and larynx cancer tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected KDM4A in NTERA-2 cells.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant fragment of human KDM4A expressed in E. Coli. 克隆 5H1 宿主 Mouse 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody 基因符号 - KDM4A
- JMJD2
- JHDM3A
- JMJD2A
- KIAA0677
纯化方法 Unpurified UniProt编号 - O75164
产品使用声明 质量保证 Evaluated by Western Blotting in HEK293 and KDM4A-hIgGFc transfected HEK293 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected KDM4A-hIgGFc transfected HEK293 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,17-10241,ChIPAb+™ Acetyl-Histone H3 (Lys9/18) - ChIP Validated Antibody and Primer Set
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