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merck millipore,默克密理博,AQ300F,Sheep Anti-Mouse Ig Antibody, F(ab')2, FITC conjugate
产品名称:Sheep Anti-Mouse Ig Antibody, F(ab')2, FITC conjugate
产品型号:AQ300F
Sheep anti-Mouse Ig Antibody, F(ab')2, FITC conjugate is an antibody against Mouse Ig for use in IF.
merck millipore,默克密理博,AQ300F,Sheep Anti-Mouse Ig Antibody, F(ab')2, FITC conjugate
- 产品介绍
描述 产品目录编号 AQ300F 品牌系列 Chemicon®
商名 - Chemicon
描述 Sheep Anti-Mouse Ig Antibody, F(ab')2, FITC conjugate 概述 The reagent is an anti-mouse Ig antibody, F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin digestion and conjugated with 5(6)- carboxy-fluorescein-N-hydroxysuccinimide ester (fluorescein) and stabilized in buffer.The molar ratio of fluorescein: protein is between 3-4.
PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.产品信息 格式 FITC 演示 Lyophilized. Buffer = 0.02 M sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.1% sodium azide
RECONSTITUTION:
Reconstitute with 200 µL of sterile distilled water.应用 应用 Sheep anti-Mouse Ig Antibody, F(ab')2, FITC conjugate is an antibody against Mouse Ig for use in IF. 主要应用 - Immunofluorescence
应用说明 The reagent allows the detection of primary mouse antibodies (monoclonal/ polyclonal) in immunohistochemistry (Volson 1984), immunocytochemistry (Gonatas et al. 1987) as well as the detection of mouse monoclonal antibodies from hybridoma supernatants by indirect immunofluorescence.
RECOMMENDED CONCENTRATION:
Immunohisto/cytochemistry: 1:50 (20 μg/mL)
The concentrated stock solution (1.0 mg/mL) should be diluted with PBS, pH 7.1 with 15 mg/mL bovine serum albumin.生物信息 宿主 Sheep 特异性 The reagent recognizes all mouse immunoglobulin classes and subclasses, independently from the type of the light chain. The antibody shows no cross- reactivity (<0.01%) with fetal calf serum (FCS) and normal serum from bovine, rabbit, goat, horse, and human. Weak reaction can be detected with normal serum from guinea pig (<0.1%). Cross-reactivity can be detected with normal serum from rat (<10%).
FLUORESCEIN/PROTEIN MOLAR RATIO:
Between 3 and 4.品种反应性 Mouse 抗体类型 Polyclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 When protected from light, the lyophilized antibody-conjugate is stable at +4°C.The reconstituted antibody conjugate solution is stable for 2 months at +4°C, when protected from light.The solution should be stored in aliquots at -20°C; repeated freezing and thawing should be avoided. 包装信息 数量 200 µg
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