merck millipore,默克密理博,03-202,RIPAb+ Gemin2 - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-202,RIPAb+ Gemin2 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+ Gemin2 - RIP Validated Antibody and Primer Set
产品型号：03-202
This RIPAb+ Gemin2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-202,RIPAb+ Gemin2 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-202,RIPAb+ Gemin2 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Ch, H, M, Xn	ELISA, WB, ICC

描述
产品目录编号	03-202
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ Gemin2 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. Gemin2 (also known as SIP1 or SMN interacting protein1) is a part of the SMN complex. The SMN complex plays a role in RNA metabolism, splicing regulation and assembly of spliceosomal snRNA complexes containing U1 and U5.
Alternate Names	survival of motor neuron protein interacting protein GEMIN2 Gemin2 Component of gems SMN interacting protein 1-delta SMN-interacting protein 1 survival interacting protein 1
背景信息	Gemin-2 (also known as SIP1 or SMN interacting protein1) is a part of the SMN complex. The SMN complex plays a role in RNA metabolism and in splicing regulation. As part of the SMN complex Gemin-2 interacts directly with Gemin-5 in a heteromeric complex that includes Gemin-3, Gemin-4, Gemin-6. This macromolecular complex (SMN complex) mediates assembly of spliceosomal snRNA complexes containing U1 and U5.

产品信息
格式	Purified
控制	Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human U1snRNA.
演示	Anti-Gemin2 (Mouse Monoclonal). One vial containing 50 μg of purified mouse IgG1k in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 30% glycerol. Store at -20°C. Normal Mouse IgG. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, U1snRNA. One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C. FOR: GGG AGA TAC CAT GAT CAC GAA GGT REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

应用
应用	This RIPAb+ Gemin2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	ELISA Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG or 5 µg of Anti-Gemin2 antibody. Ten percent of the precipitated proteins (lane 1: mouse IgG, lane 3: Gemin2) and HeLa lysate (lane 2) were resolved by electrophoresis, transferred to PVDF and probed with anti-Gemin2 antibody (1:1000 dilution). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) Arrow indicates Gemin2 (Please see figures). Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with anti-Gemin2 (0.5 μg/mL dilution). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates (~32 kDa) (Please see figures).

生物信息
免疫原品种	His-tagged recombinant protein corresponding to human Gemin-2.
宿主	Mouse
特异性	Antibody recognizes Gemin-2.
同种型	IgG1κ
品种反应性	ChickenHumanMouseXenopus
Species Reactivity Note	Demonstrated to react with human. Predicted to recognize mouse based on 100% sequence homology. Xenopus (Fischer, U., et al., 1997) and chicken (Wang, J., et al., 2001) have been shown to react with this antibody.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001009182.1
Entrez基因汇总	his gene encodes one of the proteins found in the SMN complex, which consists of the suvival of motor neuron protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. [provided by RefSeq]
基因符号	SIP1-delta SIP1
纯化方法	Protein G purfied
UniProt编号	O14893
UniProt汇总	Function: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Subunit structure: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN5.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG or 5 µg of Anti-Gemin2 antibody and the Magna RIP™ RNA-Binding Protein Immuno-precipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Gemin2-associated RNA was verified by qPCR using RIP Primers U1snRNA (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG or 5 µg of Anti-Gemin2 antibody and the Magna RIP™ RNA-Binding Protein Immuno-precipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Gemin2-associated RNA was verified by qPCR using RIP Primers U1snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.