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品种反应性 主要应用 宿主 格式 抗体类型 R, Ca, H, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT42 描述 Anti-Myosin-VI Antibody Alternate Names - Unconventional myosin-6
- Myosin-VI
背景信息 Myosin-VI has been found in the membrane ruffles at the leading edge of the Golgi complex. Myosin-VI moves along the actin in the opposite way of other known myosins to date, toward the minus end. It can exist as a dimer and a monomer and is thought to function as a non-processive motor with a 18nm working stroke. Myosin-VI has also been found in the brain, specifically the synapses. Myosin-VI deficiency (Snell’s waltzer disease) in the hippocampus causes a smaller number of synapses, short dendritic spines and astrogliosis. Myosin-VI may also be important in clathrin-mediated endocytosis of acid-type glutamate receptors. Recent research has implicated myosin-VI in the enrichment of proteins on the surface of axons. 产品信息 格式 Affinity Purified 控制 - MDCK cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Myosin-VI Antibody detects level of Myosin-VI & has been published & validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry analysis: A 1:500 dilution from a representative lot detected Myosin-VI in NIH/3T3 and A431 cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Myosin-VI. 宿主 Rabbit 品种反应性 RatCanineHumanMouse Species Reactivity Note Demonstrated to react with Canine, Human, and Mouse.
Predicted to react with Rat based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004990
Entrez基因汇总 This gene encodes a protein involved intracellular vesicle and organelle transport, especially in the hair cell of the inner ear. Mutations in this gene have been found in patients with non-syndromic autosomal dominant and recessive hearing loss. [provided by RefSeq]. 基因符号 - MYO6
纯化方法 Affinity Purified UniProt编号 - Q9UM54
UniProt汇总 FUNCTION: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).
SUBUNIT STRUCTURE: Homodimer. Binding to calmodulin through a unique insert, not found in other myosins, located in the neck region between the motor domain and the IQ domain appears to contribute to the directionality reversal. This interaction occurs only if the C-terminal lobe of calmodulin is occupied by calcium. Interaction with F-actin/ACTN1 occurs only at the apical brush border domain of the proximal tubule cells (By similarity). Interacts with DAB2. In vitro, the C-terminal globular tail binds a C-terminal region of DAB2. Interacts with CFTR. Forms a complex with CFTR and DAB2 in the apical membrane of epithelial cells. Interacts with OPTN (By similarity).
SUBCELLULAR LOCATION: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Golgi apparatus (By similarity). Nucleus. Cytoplasm › perinuclear region. Membrane › clathrin-coated pit. Cell projection › ruffle membrane; Peripheral membrane protein. Note: Also present in endocyctic vesicles, and membrane ruffles. Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage. Recruited into membrane ruffles from cell surface by EGF-stimulation. Colocalizes with DAB2 in clathrin-coated pits/vesicles. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (By similarity).
Isoform 3: Cytoplasmic vesicle › clathrin-coated vesicle membrane
Isoform 4: Cytoplasmic vesicle › clathrin-coated vesicle membrane. Cell projection › ruffle membrane
TISSUE SPECIFICTY: Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells.
DOMAIN: Divided into three regions: a N-terminal motor (head) domain, followed by a neck domain consisting of a calmodulin-binding linker domain and a single IQ motif, and a C-terminal tail region with a coiled-coil and a unique globular domain required for interaction with other proteins.
PTM: Phosphorylation in the motor domain, induced by EGF, results in translocation of MYO6 from the cell surface to membrane ruffles and affects F-actin dynamics. Phosphorylated in vitro by p21-activated kinase (PAK) (By similarity).
INVOLVEMENT IN DISEASE: Defects in MYO6 are the cause of deafness autosomal dominant type 22 (DFNA22) [MIM:606346]. DFNA22 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA22 is progressive and postlingual, with onset during childhood. By the age of approximately 50 years, affected individuals invariably have profound sensorineural deafness.
Defects in MYO6 are the cause of deafness autosomal recessive type 37 (DFNB37) [MIM:607821].
Defects in MYO6 are the cause of deafness sensorineural with hypertrophic cardiomyopathy (DFNHCM) [MIM:606346].
SEQUENCE SIMILARITIES: Contains 1 IQ domain.
Contains 1 myosin head-like domain.
SEQUENCE CAUTION: The sequence BAA20843.2 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in MDCK cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Myosin-VI in 10 µg of MDCK cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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