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merck millipore,默克密理博,ABT120,Anti-Protein Regulator of Cytokinesis 1 (PRC1) Antibody
产品名称：Anti-Protein Regulator of Cytokinesis 1 (PRC1) Antibody
产品型号：ABT120
Anti-Protein Regulator of Cytokinesis 1 (PRC1) Antibody is an antibody against Protein Regulator of Cytokinesis 1 (PRC1) for use in WB & IC.
merck millipore,默克密理博,ABT120,Anti-Protein Regulator of Cytokinesis 1 (PRC1) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, R ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT120 Replaces MABE198 描述 Anti-Protein Regulator of Cytokinesis 1 (PRC1) Antibody Alternate Names - Protein regulator of cytokinesis 1
背景信息 Protein Regulator of Cytokinesis 1 (PRC1) is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1, a mitotic spindle-associated Cdk substrate that is essential to cell cleavage, is a microtubule binding and bundling protein both in vivo and in vitro. Overexpression of PRC1 extensively bundles interphase microtubules, but does not affect early mitotic spindle organization. PRC1 has been shown to down-regulate the GAP activity of MgcRac-GAP during the metaphase and thereby contributes to the correct formation of the spindle. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Protein Regulator of Cytokinesis 1 (PRC1) Antibody is an antibody against Protein Regulator of Cytokinesis 1 (PRC1) for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Protein Regulator of Cytokinesis 1 (PRC1) in metaphase and anaphase of the cell cycle in HeLa cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the Spectrin-Fold region of Protein Regulator of Cytokinesis 1 (PRC1). 表位 Spectrin-Fold region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the Spectrin-Fold region of Protein Regulator of Cytokinesis 1 (PRC1). 品种反应性 BovineHumanMouseRat Species Reactivity Note Demonstrated to react with Human. Predicted to react with Mouse, Rat, and Bovine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003972
Entrez基因汇总 This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - PRC1
纯化方法 Affinity purified UniProt编号 - O43663
UniProt汇总 FUNCTION: Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
SUBUNIT STRUCTURE: Homodimer. Interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. Interacts with KIF14 and KIF20A.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm › cytoskeleton › spindle pole. Note: Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this dilution detected Protein Regulator of Cytokinesis 1 (PRC1) in 10 µg HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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