merck millipore,默克密理博,ABS613,Anti-ISCU Antibody

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merck millipore,默克密理博,ABS613,Anti-ISCU Antibody
产品名称：Anti-ISCU Antibody
产品型号：ABS613
Detect Iron-sulfur cluster assembly enzyme ISCU using this rabbit polyclonal antibody, Anti-ISCU Antibody validated for use in western blotting, IHC & Immunofluorescence.

merck millipore,默克密理博,ABS613,Anti-ISCU Antibody

产品介绍

merck millipore,默克密理博,ABS613,Anti-ISCU Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABS613
描述	Anti-ISCU Antibody
Alternate Names	ISCU Iron-sulfur cluster assembly enzyme ISCU, mitochondrial NifU-like N-terminal domain-containing protein NifU-like protein
背景信息	The enzyme currently named Iron-sulfur cluster assembly enzyme ISCU, mitochondrial, or in earlier literature NifU-like N-terminal domain-containing protein or NifU-like protein, and encoded by the human gene named ISCU or NIFUN, is a biosynthetic enzyme involved in the assembly of iron-sulfur proteins. There are two isoforms in mammals. Isoform 1 is localizes to the mitochondria, whereas isoform 2 localizes to the cytoplasm. ISCU is expressed in a variety of tissues including brain, heart, skeletal muscle, lung and kidney. Iron-sulfur proteins are proteins characterized by the presence of iron-sulfur clusters containing sulfide-linked centers in variable oxidation states. Iron-sulfur cluster proteins are best known for their role in the oxidation-reduction reactions of mitochondrial electron transport. Both Complex I and Complex II of oxidative phosphorylation have multiple Fe-S clusters and thus require functional ISCU to exist. In addition, iron-sulfur clusters are found in a variety of metalloproteins, such as the ferredoxins, as well as NADH dehydrogenase, hydrogenases, Coenzyme Q - Cytochrome C reductase, Succinate-coenzyme Q reductase and nitrogenase. New research has demonstrated that iron-sulfur proteins also can regulate gene expression and can be regulated by nitric oxide. EMD-Millipore’s Anti-ISCU rabbit polyclonal antibody has been tested in western blots on mouse heart tissue extracts, paraffin embedded immunohistochemistry on human lung cancer sections, and in fluorescent immunocytochemistry on MCF7 cells in culture.

产品信息
格式	Affinity Purified
演示	Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.

应用
应用	Detect Iron-sulfur cluster assembly enzyme ISCU using this rabbit polyclonal antibody, Anti-ISCU Antibody validated for use in western blotting, IHC & Immunofluorescence.
主要应用	Immunohistochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ISCU in human lung cancer tissue. Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ISCU in MCF-7 cells.

生物信息
免疫原品种	GST-tagged fusion protein corresponding to human ISCU.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
基因符号	NIFUN ISCU
纯化方法	Antigen Affinity purified
UniProt编号	Q9H1K1

产品使用声明
质量保证	Evaluated by Western Blotting in mouse heart tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected ISCU in mouse heart tissue lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.