- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS437 描述 Anti-RSPO1 Antibody Alternate Names - hRspo1
- R-spondin1
- RSPO
- CRISTIN3
- Roof plate-specific spondin-1
背景信息 R-spondin-1 (RSPO1), or Roof plate-specific spondin-1 protein, is potent activator of the Wnt/beta-catenin signaling pathway leading to TCF/LEF (T cell-specific transcription factor/lymphoid enhancer factor) gene activation. RSPO1 operates on both the canonical Wnt/beta-catenin and non-canonical Wnt signaling pathways by acting as an inhibitor of ZNRF3 (zinc and ring finger 3) protein, a cell surface transmembrane E3 ubiquitin ligase, which is an important regulator of Wnt signaling. RSPO1 interacts with the extracellular domain of ZNRF3 and induces ZNRF3 membrane clearance. RSPO1 is also part of a family of secreted proteins that play critical roles in vertebrate development, including female sex development, and RSPO1 is required for early development of the gonads regardless of sex. Molecularly, RSPO1’s activities synergize with those of WNT4 and act to stabilize beta catenin which then activates its downstream targets. RSPO1 has been shown to be a potent mitogen for specific cell types such as gastrointestinal epithelium, and it is being examined as a key target for colon cancer therapy and for the treatment of mucositis (inflammation of the oral cavity, which is common among patients receiving chemotherapy or radiation for head and neck cancers). Recently RSPO1 was also shown to be a novel beta-cell line growth factor and a factor that promotes insulin secretion in a glucose independent fashion, thus making it a target for enhancing beta-cell function in patients with type 2 diabetes. Defects in RSPO1 can lead to female to male sex reversal and have been associated with the genetic disease PKKSCC (palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal). EMD Millipore’s Anti-RSPO1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human and rat samples. 产品信息 格式 Affinity Purified 控制 - Rat spleen tissue lysate
演示 Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide. 应用 应用 Detect R-spondin-1 using this rabbit polyclonal antibody, Anti-RSPO1 Antibody validated for use in western blotting, IHC & Immunofluorescence. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected RSPO1 in human spleen tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected RSPO1 in human spleen cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human RSPO1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 RatHuman 抗体类型 Polyclonal Antibody 基因符号 - RSPO1
纯化方法 Antigen Affinity purified UniProt编号 - Q2MKA7
产品使用声明 质量保证 Evaluated by Western Blotting in rat spleen tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected RSPO1 in 15 µg of rat spleen tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,SLGP033RS,Millex-GP，0.22 µm，改良聚醚砜，33 mm，射线灭菌
下一件merck millipore产品：merck millipore,默克密理博,AB3743,Anti-OSTERIX;100 µg Antibody
相关产品
merck millipore,默克密理博,ABC281,Anti-PRDX5 Antibody
merck millipore,默克密理博,07-1008,Anti-IKKβ Antibody
merck millipore,默克密理博,SF-1720,IKBKE; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,12-564,Trimethyl-Histone H3 (Lys4) Peptide, biotin conjugate
merck millipore,默克密理博,SLGP033RS,Millex-GP，0.22 µm，改良聚醚砜，33 mm，射线灭菌
merck millipore,默克密理博,AB3743,Anti-OSTERIX;100 µg Antibody
merck millipore,默克密理博,MABE204,Anti-HoxB4 Antibody, clone EP1919Y, rabbit monoclonal
merck millipore,默克密理博,MABN153,Anti-HuC/HuD Antibody, clone 15A7.1
merck millipore,默克密理博,05-417,Anti-Paxillin Antibody, clone 5H11
merck millipore,默克密理博,681636,W-13, Hydrochloride - CAS 88519-57-7 - Calbiochem