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品种反应性 主要应用 宿主 格式 抗体类型 M, H, Ca, Ch, B, Fe WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1008 描述 Anti-IKKβ Antibody Alternate Names - inhibitor of nuclear factor kappa B kinase beta subunit
- I-kappa-B kinase 2
- I-kappa-B-kinase beta
- Nuclear factor NF-kappa-B inhibitor kinase beta 2, 3 <br />inhibitor of kappa light polypeptide gene enhancer in<br />B-cells, kinase beta
背景信息 IKKβ (IkB Kinase, IKK beta, I-Kappa-B kinase-beta) is a member of the IKKcomplex which is composed of IKKalpha, IKKbeta, IKKgamma and IKAP. IkB Kinase (IKK) complex is a critical component in NFkB regulation. In a majority of unstimulated cells, the NFkB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IkB proteins. For NFkB to become activated, it must first disassociate from the inhibitor IkB. This occurs via the serine phosphorylation of IkB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFalpha, NIK, a serine/threonine kinase, becomes activated and phosphorylates IKKα and IKKβ. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKKβ autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKKβ activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IkBalpha on the two serine residues. This phosphorylation marks IkB for ubiquination and destruction by the proteosome. This free the NFkB complex and allows it to translocates to the nucleus. 产品信息 格式 Affinity Purified 控制 - MCF-7 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide. 应用 应用 This Anti-IKKβ Antibody is validated for use in WB for the detection of IKKβ. 主要应用 - Western Blotting
应用说明 Western Blot Analysis (SNAP ID Technique): 2-10 µg/mL from a previous lot worked in western blot using the SNAP ID technique. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to IKKβ. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes IKKβ. 品种反应性 MouseHumanCanineChickenBovineFeline Species Reactivity Note Proven to react with human. Predicted to react with cat, dog, chicken, cow, and mouse based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001547
Entrez基因汇总 NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) 基因符号 - NFKBIKB
- EC 2.7.11.10
- FLJ40509
- IKK-B
- IKK-beta
- IKK2
- IKKB
- IkBKB
- MGC131801
纯化方法 Affinity Purfied UniProt编号 - O14920
UniProt汇总 FUNCTION: Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
CATALYTIC ACTIVITY: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
SUBUNIT STRUCTURE: Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with Yersinia yopJ. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct.
SUBCELLULAR LOCATIION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
Ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: : 1-4 µg/mL of this antibody detected IKKβ in 10 µg of MCF-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C for 1 year from date of receipt. 包装信息 数量 150 µg
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