merck millipore,默克密理博,05-417,Anti-Paxillin Antibody, clone 5H11

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merck millipore,默克密理博,05-417,Anti-Paxillin Antibody, clone 5H11
产品名称：Anti-Paxillin Antibody, clone 5H11
产品型号：05-417
Anti-Paxillin Antibody, clone 5H11 is an antibody against Paxillin for use in IC, IP & WB.

merck millipore,默克密理博,05-417,Anti-Paxillin Antibody, clone 5H11

产品介绍

merck millipore,默克密理博,05-417,Anti-Paxillin Antibody, clone 5H11

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Av, H, R, M, B	WB, ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-417
Replaces	04-581
品牌系列	Upstate
商名	Upstate
描述	Anti-Paxillin Antibody, clone 5H11
Alternate Names	paxillin FLJ16691
背景信息	Paxillin, a focal adhesion scaffolding protein, is phosphorylated on multiple tyrosine residues in response various stimulations including integrin stimulation, growth factor stimulation, and oncogenic transformation (v-Src, v-Crk and BCR/ABL). Paxillin binds many other focal adhesion proteins including FAK, Vinculin, Talin, and Tensin. As such, Paxillin and it tyrosine phosphorylation, help regulate the dynamics of the focal adhesions and the actin cytoskeleton which results in cell morphology and cell migration characteristics of the cell. Paxillin is most notable phosphorylated on tyrosines 31 and 118 (Tyr31 and Tyr118). Interestingly, Vinculin and FAK bind to overlapping sites on Paxillin. Additionally, there is an increase in Paxillin phosphorylation at Tyr118 in vinculin (-/-) and Vinculin Y822F cells. Paxillin Y31F Y118F expression restores apoptosis and inhibits Erk activity.

产品信息
格式	Purified
控制	Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示	Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C.

应用
应用	Anti-Paxillin Antibody, clone 5H11 is an antibody against Paxillin for use in IC, IP & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated paxillin from 500 μg of a 3T3/A31 RIPA lysate. Immunocytochemistry: 5-10 μg/mL of a previous lot gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes.

生物信息
免疫原品种	GST fusion protein containing fulllength human paxillin. Clone 5H11.
克隆	5H11
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes paxillin.
同种型	IgG1
品种反应性	AvianHumanRatMouseBovine
Species Reactivity Note	Human, mouse, rat, chicken and bovine. Other species cross-reactivity not tested.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002859
基因符号	FLJ16691 PXN Paxillin paxillin
纯化方法	Protein G purfied
UniProt编号	P49023
UniProt汇总	FUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). SIZE: 591 amino acids; 64533 Da SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5. PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. SIMILARITY: SwissProt: P49023 ## Belongs to the paxillin family. & Contains 4 LIM zinc-binding domains.

产品使用声明
质量保证	Routinely evaluated by Western Blot on RIPA lysates from 3T3/A31 fibroblasts. Western Blot Analysis: 0.5-2 μg/mL of this lot detected paxillin in RIPA lysates from 3T3/A31 fibroblasts.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.