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merck millipore,默克密理博,ABS229,Anti-HMG-CoA reductase Antibody
产品名称：Anti-HMG-CoA reductase Antibody
产品型号：ABS229
Anti-HMG-CoA reductase Antibody detects level of HMG-CoA reductase & has been published & validated for use in Immunoprecipitation, Western Blotting.
merck millipore,默克密理博,ABS229,Anti-HMG-CoA reductase Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS229 Replaces 07-572 描述 Anti-HMG-CoA reductase Antibody Alternate Names - HMG-CoA reductase
- 3-hydroxy-3-methylglutaryl-coenzyme A reductase
背景信息 The 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) enzyme is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum (ER), but also projects an active C-terminal catalytic tail into the cytoplasm. It is the rate-limiting enzyme in the mevalonate pathway as it catalyzes the conversion of HMG CoA to mevalonate, which is a critical precursor protein in the synthesis of sterols such as cholesterols. In mammalian cells, HMG-CoA reductase is regulated by multiple mechanisms. It is downregulated by high levels of exogenous cholesterol bound to the low density lipoprotein. It is also regulated by sterol and nonsterol metabolites of mevalonate which may exert inhibitory effects at the transcriptional level. Previous studies have suggested that sterols may inhibit the sterol regulatory element-binding proteins (SREBPs) which function as transcription factors that enhance the expression of genes required for sterol biosynthesis. The end-stage degradation process may be mediated by the ubiquitin degradation system. The inhibition of HMG-CoA reductase has been widely studied for the treatment of cholesterol-related conditions. 产品信息 格式 Affinity Purified 控制 - HepG2 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-HMG-CoA reductase Antibody detects level of HMG-CoA reductase & has been published & validated for use in Immunoprecipitation, Western Blotting. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 10 µg/mL from a representative lot immunoprecipitated HMG CoA reductase from HepG2 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the linker domain of human HMG CoA reductase. 表位 Linker domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes HMG CoA reductase at the linker domain. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. Predicted to react with Primate based on 100% sequence homology. Other homologies: Porcine (85% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000850
基因符号 - HMGCR
纯化方法 Affinity purified UniProt编号 - P04035
UniProt汇总 FUNCTION: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
ENZYME REGULATION: Inhibited by statins, a class of hypolipidemic agents used as pharmaceuticals to lower cholesterol levels in people at risk for cardiovascular disease because of hypercholesterolemia. Inhibition of HMGCR in the liver stimulates the LDL-receptors, which results in an increased clearance of LDL from the bloodstream and a decrease in blood cholesterol levels. The first results can be seen after one week of use and the effect is maximal after four to six weeks.
PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
SUBUNIT STRUCTURE: Homodimer.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane; Multi-pass membrane protein.
SEQUENCE SIMILARITIES: Belongs to the HMG-CoA reductase family.产品使用声明 质量保证 Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected HMG CoA reductase on 10 µg of HepG2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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