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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS186 描述 Anti-Spred-1 Antibody Alternate Names - hSpred1
- Sprouty-related, EVH1 domain-containing protein 1
- Spred-1
背景信息 Spred1 is a tyrosine kinase substrate which has a cysteine-rich domain which resembles the SPR domain at the carboxy terminus. Spred1 associated with Ras, blocks phosphorylation of Raf and disrupts MAP kinase signaling during the differentiation of neuronal cells and myocytes. A mutation in Spred1 has been linked to Lugius syndrome, an autosomal dominant RAS-MAPK pathway syndrome. 产品信息 格式 Affinity Purified 控制 - HEK293 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Spred-1 Antibody is an antibody against Spred-1 for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Spred-1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human and Mouse. Predicted to react with Rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_689807
Entrez基因汇总 The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq]. 基因符号 - SPRED1
纯化方法 Affinity purified UniProt编号 - Q7Z699
UniProt汇总 FUNCTION: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).
SUBUNIT STRUCTURE: Interacts with Ras (By similarity). Homodimer and heterodimer. Interacts with CAV1. Able to interact with SPRED2 to form heterodimers.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Membrane › caveola; Peripheral membrane protein. Nucleus. Note: Localized in cholesterol-rich membrane raft/caveola fractions.
TISSUE SPECIFICITY: Weakly expressed in embryonic cell line (HEK-293).
PTM: Phosphorylated on tyrosine (By similarity).
INVOLVEMENT IN DISEASE: Defects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
SEQUENCE SIMLARITIES: Contains 1 KBD domain.
Contains 1 SPR (sprouty) domain.
Contains 1 WH1 domain.
SEQUENCE CAUTION: The sequence AAH18015.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.产品使用声明 质量保证 Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Spred-1 on 10 µg of HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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