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品种反应性 主要应用 宿主 格式 抗体类型 B, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS173 描述 Anti-phospho-RalB Antibody (Ser198) Alternate Names - Ras-related protein Ral-B
背景信息 RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorgenesis by driving exocyst assembly and polarizatied exocystosis in epithelaial cells. RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration. Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. 产品信息 格式 Affinity Purified 控制 - Non-phospho and phospho-RalB peptides
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect the phospho-RalB (Ser198) protein using this Anti-phospho-RalB (Ser198) validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-RalB (Ser198), but does not detect phospho-RalA or mutant phospho-RalB (Ser198A) in transfected 293T cell lysates.
Western Blot Analysis: A 1;1,000 dilution from representative lot detected WT phospho-RalB (Ser198) in untransfected SW480 cell lysates, but demonstrates a loss of signal in shRalB transfected SW480 cell lysates.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human RalB phosphorylated at Ser198. 表位 Phosphorylated Ser198 宿主 Rabbit 特异性 This antibody recognizes phospho-RalB (Ser198), but does not cross-react with RalA or mutant phospho-RalB (Ser198A). 品种反应性 BovineHuman Species Reactivity Note Demonstrated to react with Human. Predicted to react with Bovine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002872
基因符号 - RALB
纯化方法 Affinity purified UniProt编号 - P11234
产品使用声明 质量保证 Evaluated by Dot Blot in non-phospho and phospho-RalB peptides.
Dot Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-RalB peptide in Dot Blot.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,ABS45,Anti-phospho-MYPT1 (Thr696) Antibody
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