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merck millipore,默克密理博,07-1353,Anti-acetyl-lysine EPOR (Lys373) Antibody
产品名称：Anti-acetyl-lysine EPOR (Lys373) Antibody
产品型号：07-1353
Anti-acetyl-lysine EPOR (Lys373) Antibody is an antibody against acetyl-lysine EPOR (Lys373) for use in WB.
merck millipore,默克密理博,07-1353,Anti-acetyl-lysine EPOR (Lys373) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, H, M, B, Ca WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1353 描述 Anti-acetyl-lysine EPOR (Lys373) Antibody Alternate Names - EPO-R
- Erythropoietin receptor
背景信息 EPOR is the receptor for erythropoietin and high expression has been associated with extended erythroblast proliferation and differentiation. EPOR and KIT work together to ensure normal erythroid cell expansion. EPOR has recently been found to interact will membrane-acting androgens to protect tumor cells from apoptosis. 产品信息 格式 Affinity Purified 控制 - Sodium butyrate untreated and treated HeLa cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-acetyl-lysine EPOR (Lys373) Antibody is an antibody against acetyl-lysine EPOR (Lys373) for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human EPOR acetylated at Lys373. 表位 Acetylated Lys373 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes EPOR acetylated at Lys373. 品种反应性 PigHumanMouseBovineCanine Species Reactivity Note Demonstrated to react with Human. Predicted to react with Mouse, Bovine, Canine, and Porcine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000112
Entrez基因汇总 This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants. 基因符号 - EPOR
修改 - Acetylation
纯化方法 Affinity purified UniProt编号 - P19235
UniProt汇总 FUNCTION: Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
SUBUNIT STRUCTURE: Forms homodimers on EPO stimulation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins including LYN By similarity, the adapter protein APS, PTPN6 (By similarity), PTPN11, JAK2, PI3 kinases, STAT5A/B, SOCS3, CRKL By similarity. Interacts with INPP5D/SHIP1 (By similarity). The N-terminal SH2 domain of PTPN6 binds Tyr-454 and inhibits signaling through dephosphorylation of JAK2 (By similarity). APS binding also inhibits the JAK-STAT signaling. Binding to PTPN11, preferentially through the N-terminal SH2 domain, promotes mitogenesis and phosphorylation of PTPN11 (By similarity). Binding of JAK2 (through its N-terminal) promotes cell-surface expression (By similarity). Interaction with the ubiquitin ligase NOSIP mediates EPO-induced cell proliferation. Interacts with ATXN2L.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein
Isoform EPOR-S: Secreted. Note: Secreted and located to the cell surface.
TISSUE SPECIFICITY: Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
DOMAIN: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
PTM: On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
INVOLVEMENT IN DISEASE: Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
SEQUENCE SIMILARITIES: Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 1 fibronectin type-III domain.产品使用声明 质量保证 Evaluated by Western Blot in sodium butyrate untreated and treated HeLa cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected EPOR on 10 µg of sodium butyrate untreated and treated HeLa cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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