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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 ABS159 描述 Anti-HPK1 Antibody Alternate Names - MEKKK 1
- Mitogen-activated protein kinase kinase kinase kinase 1
- Hematopoietic progenitor kinase
- MAPK/ERK kinase kinase kinase 1
- MEK kinase kinase 1
背景信息 Hematopoietic progenitor kinase 1 (HPK1), otherwise known as MEKKK 1 and MAP4K1, is a serine/threonine kinase belonging to the Ste20 (Sterile-20) family. It is predominantly expressed in hematopoietic cells and is involved in sigaling pathways requiring MAP kinase activation; HPK1 directly upstream of MEKK1. HPK1 forms signaling complexes with a number of adaptor proteins including Nck, SLP-76, and Grb2 family of proteins; these proteins may facilitate the tethering of HPK1 to membrane receptors such as the epidermal growth factor receptor. However, HPK1 may also interact with adaptor proteins to induce transcription. For example, interaction with CRKL, and Crk adaptor proteins activates the c-Jun N-terminal kinase (JNK) pathway leading to gene expression. HPK1 may mediate cell growth, cytokine signaling, and cell death. 产品信息 格式 Purified 控制 - Raji cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-HPK1 Antibody detects level of HPK1 & has been published & validated for use in Western Blotting, IHC(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected HPK1 in human tonsil tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of human HPK1. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes HPK1 at the N-terminus. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. Other homologies: Mouse and Rat (86% sequence homoloogy). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_009112
基因符号 - HPK1
- MAP4K1
纯化方法 Protein A purified UniProt编号 - Q92918
UniProt汇总 FUNCTION: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
SUBUNIT STRUCTURE: Interacts with MAP3K1.
TISSUE SPECIFICITY: Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected HPK1 on 10 µg of Raji cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,365251,Gö 6983 - CAS 133053-19-7 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,14-522,Abl (T315I) Protein, active, 10 µg
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