merck millipore,默克密理愽
  • merck millipore,默克密理博,HTS089M,ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

  • merck millipore,默克密理博,HTS089M,ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

    产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor
    产品型号：HTS089M
    Human LPA1 / EDG2 GPCR membrane preparation for GTPγS binding Assays.

    merck millipore,默克密理博,HTS089M,ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

  • 产品介绍
  • merck millipore,默克密理博,HTS089M,ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

    重要规格表

    应用说明 品种 宿主细胞 蛋白靶
    GTPγS binding assayHumanChem-1LPA1 / EDG2
    描述
    产品目录编号 HTS089M
    商名
    • Chemicon
    • ChemiScreen
    描述 ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor
    概述 Human EDG2 encoding LPA1
    背景信息 Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA1 is widely expressed, with particularly notable expression in the ventricular zone of the embryonic cerebral cortex. Mice lacking LPA1 exhibit craniofacial abnormalities and defective development of the cerebral cortex (Harrison et al., 2003; Choi et al., 2008). LPA1 also promotes fibrotic responses to tissue injury (Tager et al., 2008)

    Millipore's LPA1 Membrane Preparation is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for Millipore’s LPA1 Calcium Optimized Cell Line (HTS089C). This LPA1 Membrane Preparation exhibits both constitutive and agonist-induced activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist, inverse agonist, and antagonist activities. The membrane preparations exhibits EC50 of 73 nM for LPA in a GTPγS binding assay.
    产品信息
    演示 One package contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific LPA-stimulated [35S]-GTPγS binding.
    Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
    Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
    应用
    应用 Human LPA1 / EDG2 GPCR membrane preparation for GTPγS binding Assays.
    应用说明 GTPγS binding assay
    生物信息
    品种 Human
    Entrez基因编号
    • NM_001401
    GPCR类别 A
    宿主细胞 Chem-1
    蛋白靶 LPA1 / EDG2
    靶子系列 Lysophospholipid
    孵化条件 GTPγS ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/10 μM GDP in a nonbinding 96-well plate. Unlabeled ligand was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
    产品使用声明
    质量保证 1 unit = 5 μg
    EC50 in GTPγS binding assay by LPA: ~ 73 nM
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
    包装信息
    数量 200 units

    merck millipore,默克密理博,HTS089M,ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

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