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品种反应性 主要应用 宿主 格式 抗体类型 M, R ICC, WB, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN58 描述 Anti-phospho MAP1B (Thr1265) Antibody Alternate Names - MAP1 light chain LC1
- Microtubule-associated protein 1B
- MAP-1B
- MAP1(X)
- MAP1.2
背景信息 Microtubule-associated protein 1B (MAP1B) is neuron-specific and is thought to play a part in controlling the neuronal cytoskeleton. MAP1B is made up of heavy and light chains and is involved in the microtubule and actin filament association. MAP1B may have an important role in axon formation by supporting microtubule dynamics and controlling cytoskeletal arrangement. MAP1B has also been associated with Netrin 1 signaling. 产品信息 格式 Affinity Purified 控制 - S1 rat brain tissue lysate
演示 Purified rabbit polyclonal in PBS containing 0.05% sodium azide. 应用 应用 Anti-phospho MAP1B (Thr1265) Antibody is an antibody against phospho MAP1B (Thr1265) for use in WB, IH & IC. 主要应用 - Immunocytochemistry
- Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: 1:600 dilution from a representative lot detected MAP1B in embryonic (E12) rat spinal cord tissue. (Image courtesy of Dr. Phillip R. Gordon-Weeks, King's College London.)
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in IF. (Trivedi, N., et al. (2005). Journal of Cell Science. 118:993-1005.)生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to mouse MAP1B phosphorylated at Thr1265. 表位 Phosphorylated Thr1265 宿主 Rabbit 特异性 This antibody recognizes MAP1B phosphorylated at Thr1265. 品种反应性 MouseRat Species Reactivity Note Demonstrated to react with Rat. Predicted to react with Mouse based on immunogen design. Other homologies: Human (91% sequence homology). 抗体类型 Polyclonal Antibody 基因符号 - Mtap5
- Map1b
- Mtap1b
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - P14873
UniProt汇总 FUNCTION: The function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules.
SUBUNIT STRUCTURE: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. LC1 interacts with the amino-terminal region of MAP1B. Interacts with ANP32A and TIAM2. Interacts (via C-terminus) with GAN (via Kelch domains).
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton Probable.
DOMAIN: Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
PTM: LC1 is coexpressed with MAP1B. It is a polypeptide generated from MAP1B by proteolytic processing. It is free to associate with both MAP1A and MAP1B. It interacts with the N-terminal region of MAP1B.
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).
SEQUENCE SIMLARITIES: Belongs to the MAP1 family.产品使用声明 质量保证 Evaluated by Western Blot in S1 rat brain tissue lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected MAP1B on 10 µg of S1 rat brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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