- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,FCIM025162,FlowCellect™ Mouse Th2 Differentiation Tool Kit
产品名称：FlowCellect™ Mouse Th2 Differentiation Tool Kit
产品型号：FCIM025162
The FlowCellect Mouse Th2 Differentiation Tool Kit for Flow Cytometry includes a CD3 Coated Activation Plate, complete Differentiation Media & Activator/Re-Stimulation Reagents.
merck millipore,默克密理博,FCIM025162,FlowCellect™ Mouse Th2 Differentiation Tool Kit
- 产品介绍
描述 产品目录编号 FCIM025162 商名 - FlowCellect
描述 FlowCellect™ Mouse Th2 Differentiation Tool Kit 概述 The FlowCellect Mouse™ Th2 Differentiation Tool Kit includes a CD3 Coated Activation Plate, complete Differentiation Media and Activator/Re-Stimulation Reagents. Detailed assay instructions are included to assist in three culture steps: Activation, Expansion and Re-Stimulation to complete Th2 cell genaration in 6 days.
Day 1 = Activation: Culture CD4+ cells with growth factors and TCR co-stimulator on an anti-CD3 coated 96-well plate for 3 days.
Day 4 = Expantion: Expand activated/developing Th2 cells in a larger tissue culture plate.
Day 6 = Re-stimulation: Differentiated Th2 cells are re-stimulated with mitogens to induce Th2 signature cytokine production on day 6.Alternate Names - Flow Cellect
背景信息 CD4+ T cells play a central role in the function of the immune system. They help B cells produce antibodies, enhance and maintain responses of CD8+ T cells, regulate macrophage function, orchestrate immune responses against a wide variety of pathogenic microorganisms, and regulate immune responses.
Effector T cells are derived from naïve CD4+ progenitor T cells, after maturational complex interactions with antigen-presenting cells in a permissive milieu, including antigenic type and load, and cytokine signaling. Committed CD4+ T cells can differentiate into four major lineages: Th1, Th2, Th17 and regulatory T cells with distinct cytokine products and biological functions.
Th2 cells play a central role in the development of allergic responses and imnune responses against helminthic parasites. The differentiation of Th2 cells from naïve CD4+ cells in vitro requires both the activation of IL-4 receptor and IL-2 receptor signaling pathways.产品信息 部件 - 5. Re-Stimulation Reagent: (Part No.CS206520) One vial containing 48 μL cocktail of PMA and ionomycin in DMSO
- 1. Mouse CD3 Coated Activation Plate: (Part No. CS206556) One 96-well plate with strips.
- 2. Th2 Activator #1: (Part No. CS206525) One vial containing 33 µL of neutralizing antibodies.
- 3. Differentiation Media: (Part No. CS206524) One vial containing 200 mL of complete Th1 differentiation culture media
- 4. Th2 Activator #2: (Part No.CS206529) One vial containing lyophilized growth factors
应用 应用 The FlowCellect Mouse Th2 Differentiation Tool Kit for Flow Cytometry includes a CD3 Coated Activation Plate, complete Differentiation Media & Activator/Re-Stimulation Reagents. 主要应用 - Flow Cytometry
生物信息 宿主 Mixed Species 品种反应性 Mouse 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 4-8°C for antibodies and buffers 包装信息 数量 12 Tests / Kit
上一件merck millipore产品：merck millipore,默克密理博,RECYTMAG-65K,MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
下一件merck millipore产品：merck millipore,默克密理博,555555,Rho Kinase Inhibitor V - CAS 1072906-02-5 - Calbiochem
相关产品
merck millipore,默克密理博,537075,(±)-Propranolol, Hydrochloride - CAS 3506-09-0 - Calbiochem
merck millipore,默克密理博,AP194C,Donkey Anti-Chicken IgY Antibody, Cy3 conjugate, Species Adsorbed
merck millipore,默克密理博,07-1520,Anti-phospho-Grb10 (Ser501/Ser503) Antibody
merck millipore,默克密理博,69704,S-protein Agarose
merck millipore,默克密理博,555555,Rho Kinase Inhibitor V - CAS 1072906-02-5 - Calbiochem
merck millipore,默克密理博,NA19L,Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20)
merck millipore,默克密理博,SF-2632,PRL; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,ABN58,Anti-phospho MAP1B (Thr1265) Antibody