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品种反应性 主要应用 宿主 格式 抗体类型 Rhesus Macaque, M, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN117 描述 Anti-PDE4D3 Antibody Alternate Names - PDE43
- cAMP-specific 3',5'-cyclic phosphodiesterase 4D
- DPDE3
背景信息 Phosphodiesterase 4D3 (PDE4D3) is a member of the PDE cyclic nucleotides. PDE4D3 is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D3 is a direct target of the MSH/cAMP/MITF pathway and may play a role in melanocyte differentiation. PDE4D3 is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D3 may be associated with analgesic and anti-inflammatory effects. PDE4D3 has also been linked to the risk of stroke, and in particular to polygenic stroke. 产品信息 格式 Affinity Purified 控制 - Mouse brain membrane tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-PDE4D3 Antibody is validated for use in WB for the detection of PDE4D3. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of human PDE4D3. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes PDE4D3 at the N-terminus. 品种反应性 Rhesus MacaqueMouseRat Species Reactivity Note Demonstrated to react with Mouse and Rat. Predicted to react with Rhesus Monkey based on 100% sequence homology. Predicted to react with Human based on immunogen design. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006194
Entrez基因汇总 This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins. 基因符号 - DPDE3
- PDE4D
纯化方法 Affinity purified UniProt编号 - Q08499
UniProt汇总 FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid.
PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Cytoplasm › cytoskeleton (By similarity). Cytoplasm › cytoskeleton › centrosome (By similarity). Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity).
TISSUE SPECIFICTY: Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
PTM: Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
INVOLVEMENT IN DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. Ref.16 states that association with stroke has to be considered with caution.
SEQUENCE SIMILARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.产品使用声明 质量保证 Evaluated by Western Blot in mouse brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PDE4D3 on 10 µg of mouse brain membrane tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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