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品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE367 描述 Anti-RNF168 Antibody Alternate Names - RNF168
- E3 ubiquitin-protein ligase RNF168
- hRNF168
- RING finger protein 168
背景信息 E3 ubiquitin-protein ligase RNF168 (UniProt Q8IYW5; also known as hRNF168, RING finger protein 168) is encoded by the RNF168 gene (Gene ID 165918) in human. Ubiquitylation/Ubiquitination is an essential early signal in the DNA damage repair process. Multiple E3 ligases, including RNF2 (RING2), RNF8 and RNF168, mediate the addition of K63-linked polyubiquitin chains to histone 2A (H2A) and H2AX, which in turn recruits DNA damage repair proteins, e.g., RAP80, BRCA1. In addition, SUMOylation and NEDDylation are also involved in the DNA damage response. RNF168 is a dual specificity E3 ligase that mediates both the ubiquitination and NEDDylation of H2A and H2AX. The two types of modifications compete against each other and NEDD8 modification of H2A and H2AX blocks the recruitment of BRCA1 at DNA damage repair sites. RNF168 itself is also subjected to NEDD8 modification and NEDDylation of RNF168 is necessary for its ubiquitin ligase activity. Inhibition of RNF168 NEDDylation impairs its interaction with E2 enzyme Ubc13 (UBE2N). Likewise, downregulating RNF168 NEDDylation by the deNEDDylating enzyme NEDP1, or due to mutations of the NEDD8-conjugating enzyme UBC12, decreases H2A and H2AX ubiquitylation. RNF168 Mutations are linked to RIDDLE (radiosensitivity, immunodeficiency dysmorphic features and learning difficulties) syndrome. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-RNF168 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IF to detect RNF168 also known as E3 ubiquitin-protein ligase RNF168. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected RNF168 in HeLa, A431, HUVEC, and NIH/3T3 cells. 生物信息 免疫原品种 GST-tagged recombinant human RNF168. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody detects RNF168 in a lymphoblastoid cell line (LCL) derived from a healthy donor, but not LCL derived from a RIDDLE (radiosensitivity, immunodeficiency dysmorphic features and learning difficulties) syndrome patient. 品种反应性 MouseHuman Species Reactivity Note Human, Mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_689830
Entrez基因汇总 This gene encodes an E3 ubiquitin ligase protein that contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The protein is involved in DNA double-strand break (DSB) repair. Mutations in this gene result in Riddle syndrome. [provided by RefSeq, Sep 2011]. 基因符号 - RNF168
纯化方法 Affinity purified UniProt编号 - Q8IYW5
UniProt汇总 FUNCTION: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and ubiquitinates histone H2A and H2AX, leading to amplify the RNF8-dependent H2A ubiquitination and promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Interacts with UBE2N/UBC13.
SUBCELLULAR LOCATION: Nucleus. Note: Localizes to sites of DNA damage.
DOMAIN: The MIU motifs (motif interacting with ubiquitin) mediate the interaction with ubiquitin and the localization at sites of DNA damage.
INVOLVEMENT IN DISEASE: Defects in RNF168 are the cause of Riddle syndrome (RIDDLES) [MIM:611943]. Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions.
SEQUENCE SIMILARITIES: Belongs to the RNF168 family.
Contains 1 RING-type zinc finger.
SEQUENCE CAUTION: The sequence BAB70801.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC04060.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blotting in SA13 human B cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RNF168 in 8 µg of SA13 human B cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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