- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rhesus Macaque, H, M, Mk, R, B, Po, Ht ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE36 描述 Anti-RAR-alpha Antibody Alternate Names - Nuclear receptor subfamily 1 group B member 1
- Retinoic acid receptor alpha
- RAR-alpha
背景信息 Retinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ. 产品信息 格式 Affinity Purified 控制 - COS-7 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-RAR-alpha Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect RAR-alpha also known as Retinoic acid receptor alpha. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected RAR-alpha in NIH/3T3, A431 and HeLa cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human RAR-alpha. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Rhesus MacaqueHumanMouseMonkeyRatBovinePigHamster Species Reactivity Note Demonstrated to react with Human, Mouse, and Monkey. Predicted to react with Rat, Bovine, Porcine, Hamster, Rhesus Macaque, Equine, Rabbit, Chimpanzee, Orangutan, Northern White-cheeked Gibbon, and Canine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000955
Entrez基因汇总 Summary: This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus. 基因符号 - NR1B1
- RARA
纯化方法 Affinity purified UniProt编号 - P10276
UniProt汇总 FUNCTION: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (by similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.
SUBUNIT STRUCTURE: Heterodimer; with RXRA. Binds DNA preferentially as a heterodimer. Interacts with CDK7 (by similarity). Interacts with coactivators NCOA3 and NCOA6. Interacts with NCOA7; the interaction requires ligand-binding. Interacts with MLL5. Interacts (via the ligand-binding domain) with PRAME; the interaction is ligand (retinoic acid)-dependent. Interacts with AKT1; the interaction phosphorylates RARA and repressses transactivation. Interacts with PRKAR1A; the interaction negatively regulates RARA transcriptional activity. Interacts with NCOR1 and NCOR2. Interacts with PRMT2.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.
DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
POST-TRANSLATIONAL MODIFICATION: Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (by similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling. Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity. Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation. Ubiquitinated.
INVOLVEMENT IN DISEASE: Note=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.
SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
SEQUENCE CAUTION: The sequence AAB00112.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB00113.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB62809.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in COS-7 cell lysate.
Western Blot Analysis: 3 μg/mL of this antibody detected RAR-alpha on 10 µg of COS-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,SF-3619,AXIN2; Human, Cyanine 5 RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,PC635,PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb
相关产品
merck millipore,默克密理博,ABS13,Anti-TRABID Antibody
merck millipore,默克密理博,SF-194,S100B; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,09-482,Anti-PI3 Kinase Antibody, p110β
merck millipore,默克密理博,SF-3619,AXIN2; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,PC635,PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb
merck millipore,默克密理博,ABE1403,Anti-Cdk1/cdc2 Antibody C-term
merck millipore,默克密理博,69753,pET-25b(+) DNA - Novagen
merck millipore,默克密理博,MABN1080,Anti-CD35, clone EPR6601, Rabbit Monoclonal Antibody
merck millipore,默克密理博,MABF334,Anti-IL-2 (human) Antibody, FITC, clone MQ1-17H12