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品种反应性 主要应用 宿主 格式 抗体类型 R, H, Mk, Ca, M ELISA, WB, ICC, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-482 Replaces 04-399 描述 Anti-PI3 Kinase Antibody, p110β Alternate Names - phosphoinositide-3-kinase, catalytic, alpha polypeptide
- PI3-kinase p110 subunit alpha
- phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
- PtdIns-3-kinase p110
- phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
- phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic<br />subunit, alpha isoform
背景信息 PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival, signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway. 产品信息 格式 Affinity Purified 控制 - HEK-293 cell lysate.
演示 Antigen affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl, with 0.05% NaN3. 应用 应用 Anti-PI3 Kinase Antibody, p110β is an antibody against PI3 Kinase for use in WB, IP, IC, IH, ELISA. 主要应用 - ELISA
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 ELISA:
This antibody was tested and determined to work for ELISA at 0.5 ng/well.
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110β. 表位 Internal Domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes the internal domain of PI3 Kinase, p110β. 品种反应性 RatHumanMonkeyCanineMouse Species Reactivity Note Human. Expected to react with Monkey, Dog, Rat and Mouse based on immunogen sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006210.1
基因符号 - p110-alpha
- EC 2.7.1.153
- MGC142161
- MGC142163
- PI3K
纯化方法 Affinity Purfied UniProt编号 - P42336
UniProt汇总 FUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.
SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 By similarity.
INVOLVEMENT IN DISEASE: Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are associated with breast cancer [MIM:114480].
Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550]. Ref.14
PI3KCA mutations affecting exons 9 and 20 display gender- and tissue-specific patterns, thus suggesting that the different amino acid changes could exert distinct functional effects on the oncogenic properties of this enzyme. Furthermore, sexual dimorphisms and tissue specific factors might directly or indirectly influence the occurrence of PI3KCA cancer alleles.
SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.
Contains 1 C2 domain.
Contains 1 PI3K/PI4K domain.
产品使用声明 质量保证 Evaluated by Western Blot on HEK-293 cell lysate.
Western Blot Analysis: 0.05 - 0.1 μg/mL of this antibody detected endogenous PI3 Kinase, p110β in lysate from HEK-293 cells.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 100 µL
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