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品种反应性 主要应用 宿主 格式 抗体类型 R, H IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE103 描述 Anti-SIRT7 Antibody Alternate Names - SIR2-like protein 7
- NAD-dependent deacetylase sirtuin-7
背景信息 The silent information regulator (SIR) genes (sirtuins) are a highly conserved family of proteins, which contain one or more sirtuins present in various species from bacteria to mammals. There are seven sirtuin genes that encode seven sirtuin enzymes, SIRT1 through SIRT7, all of which are dependent on oxidized nicotinamide adenine dinucleotide (NAD+). These proteins serve as mono-ADP-ribosyltransferases and deacetylate histones as a means of silencing specific chromatin regions. Further research is still required to elicidate the link between its effects on cellular metabolism, stress resistance, and cellular proliferation and its proposed role in mediating general lifespan. It is reported that Sirt7 expression demonstrates an anti-proliferative role with tumorigenic potential, in several murine cell lines. 产品信息 格式 Affinity Purified 控制 - Human liver tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-SIRT7 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect SIRT7 also known as NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:00 dilution from a representative lot detected the expressed protein of SIRT7 in human liver tissue lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of the expressed protein of human SIRT7. 表位 N-terminus. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the N-terminus of the expressed protein of SIRT7. 品种反应性 RatHuman Species Reactivity Note Demonstrated to react with Human and Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_057622.1.
Entrez基因汇总 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. 基因符号 - SIR2L7
- SIRT7
纯化方法 Affinity purified UniProt编号 - Q9NRC8
UniProt汇总 FUNCTION: NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex.
CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.
COFACTOR: Binds 1 zinc ion per subunit.
SUBUNIT LOCATION: Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
产品使用声明 质量保证 Evaluated by Western Blot in human liver tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected the expressed protein of SIRT7 in 10 µg of human liver tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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