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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB, ICC Rb Serum Polyclonal Antibody 描述 产品目录编号 ABD69 Replaces AB5922 描述 Anti-Nestin, Human Antibody Alternate Names - Nestin
背景信息 Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma. 产品信息 格式 Serum 控制 - U251 glioblastoma cells.
演示 Rabbit polyclonal serum with 0.05% sodium azide. 应用 应用 Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human Nestin. 宿主 Rabbit 特异性 This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. Antibody is not reactive with rat and mouse Nestin protein. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006608
基因符号 - nestin
- FLJ21841
- Nbla00170
- OTTHUMP00000032198
纯化方法 Unpurified UniProt编号 - P48681
产品使用声明 质量保证 Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
包装信息 数量 50 µL
上一件merck millipore产品：merck millipore,默克密理博,MAB1955,Anti-Integrin α4 Antibody, clone P4C2
下一件merck millipore产品：merck millipore,默克密理博,234403,Complement C3-Depleted Serum, Human
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