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merck millipore,默克密理博,FCCH100171,FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests)
产品名称：FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests)
产品型号：FCCH100171
This FlowCellect Autophagy LC3 Antibody-based Assay Kit provides a quantitative solution for the study of autophagy & potency evaluation of autophagy inducers using flow cytometry.
merck millipore,默克密理博,FCCH100171,FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests)
- 产品介绍
描述 产品目录编号 FCCH100171 商名 - FlowCellect
描述 FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests) 概述 Millipore’s FlowCellect™ Autophagy LC3 Antibody-based Assay Kit provides a quantitative solution for the study of autophagy and potency evaluation of autophagy inducers using flow cytometry. This kit contains two key detection reagents to help facilitate the monitoring of lipidated LC3-II in a given cell system:
1) The use of selective permeabilization solution discriminates between cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins, while protecting LC3 which has been sequestered into the autophagosome;
2) Since autophagy is a constitutive cellular degradation process, the use of an autophagy detection reagent (Autophagy Reagent A) will prevent the lysosomal degradation of LC3, allowing its quantification by flow cytometry;
By having the ability to measure and quantify autophagy, we are able to screen and rank order autophagy inducers or inhibitors, monitor cell culture health and protein turnover rate, study the mechanisms of protein degradation, and identity new autophagy targets and pathways leading to aging and neurodegenerative diseases.
The anti-LC3 FITC conjugate and autophagy enabling reagents are optimized on guava® bench top flow cytometers and provide a complete solution for autophagy analysis. This kit can be used on any flow cytometer or imaging device by following the same protocol, offering researchers a reliable, quantitative, and fully validated solution to study autophagy. The anti-LC3 antibody provided in the kit has been carefully evaluated to ensure optimal performance, alleviating the need for any additional validation of the kit reagents.Alternate Names - FlowCellect
背景信息 Autophagy is an intracellular catabolic pathway that causes cellular protein and organelle turnover, and it is associated in diverse diseases including Alzheimer's disease, aging, cancers, and Crohn's disease. It is a tightly regulated process that plays a normal part in cell growth, development, and cellular homeostasis. Autophagy functions as a housekeeping mechanism by disposing of aging and/or dysfunctional proteins and organelles through sequestering and priming proteins for lysosomal degradation. Increasing evidence suggests that not just apoptosis, but also autophagy, can contribute to cell death and greatly influence general cell health. Malfunctions of the autophagy process are proposed to influence cell health longevity and the capability of cells to function at full capacity.
During autophagy, LC3 protein is translocated from the cytoplasm to the autophagosome where it is targeted to the lysosome for degradation. The process of autophagy can be categorized into four distinct stages (See figure 1):
1) Induction and LC3 Translocation: The process is initiated by external/internal stimuli (e.g. nutrient depletion);
2) Autophagosome formation: Unwanted cytosolic proteins and aging organelles are sequestered by a double membrane vesicle, i.e.—”autophagosome”. Formation of this vesicle is coordinated by complexes of Atg proteins (Atg5 and Atg12) that are conjugated, enabling the recruitment of LC3;
3) Lysosomal docking and fusion: LC3 protein regulates traffic between autophagosome to lysosome. (LC3-I is cytoplasmic; LC3-II is lipidated and sequestered into autophagosomal membrane);
4) Degradation: Fusion with the lysosome and subsequent breakdown of the autophagic vesicle and its contents.产品信息 部件 - 4. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL
- 1. 20X Anti-LC3 FITC, clone 4E12: (Part No. CS208214). One vial containing 600 L
- 2. Autophagy reagent A: (Part No. CS208212) One vial (lyophilized)
- 3. Autophagy reagent B: (Part No. CS208215) One vial containing 1 mL
检测方法 Fluorescent 格式 Cell Line 应用 应用 This FlowCellect Autophagy LC3 Antibody-based Assay Kit provides a quantitative solution for the study of autophagy & potency evaluation of autophagy inducers using flow cytometry. 主要应用 - Flow Cytometry
生物信息 品种反应性 Human 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 All reagents must be stored at 2 - 8ºC.
All kit components are stable up to six (6) months from date of receipt if stored and handled correctly.包装信息 数量 100 Tests
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