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品种反应性 主要应用 宿主 格式 抗体类型 M IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 ABD28 描述 Anti-Rfx6 Antibody Alternate Names - Regulatory factor X domain-containing protein 1
- DNA-binding protein RFX6
- Regulatory factor X 6
背景信息 Rfx6 (regulatory factor X 6) is a winged-helix transcription factor that is essential for development and seems to be located exclusively in the pancreatic islets. It has been associated with endoderm development, specifically, and the differentiation of islet cells. Since insulin production happens in the beta-cells of pancreatic islets, Rfx6 may be used clinically to treat diabetes. Recently, Rfx6 function has been found to change over time and gets restricted to endocrine cells at later stages. Mitchell-Riley syndrome is thought to stem from a Rfx6 mutation. 产品信息 格式 Serum 控制 - Mouse embryo pancreas tissue
演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Anti-Rfx6 Antibody is a Rabbit Polyclonal Antibody for detection of Rfx6 also known as DNA-binding protein RFX6, Regulatory factor X 6 & has been validated in IHC. 主要应用 - Immunohistochemistry
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to mouse Rfx6. 宿主 Rabbit 品种反应性 Mouse Species Reactivity Note Demonstrated to react with Mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001152861
基因符号 - Rfxdc1
- Rfx6
纯化方法 Unpurified UniProt编号 - Q8C7R7
UniProt汇总 FUNCTION: Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes.
SUBUNIT STRUCTURE: Interacts with RFX3.
SUBCELLULAR LOCAION: Nucleus
TISSUE SPECIFICTY: In the adult pancreas, expression is restricted to the islets where it could be detected in all endocrine lineages.
DEVELOPMENTAL STAGE: Expressed initially broadly in the definitive endoderm after gastrulation, becomes restricted to the gut and pancreatic bud at mid gestation, and is reactivated by Neurog3 in islet progenitor cells and is ultimately restricted to pancreatic islets in the mature pancreas (at protein level).
INDUCTION: By Neurog3.
DISRUPTION PHENOTYPE: Mice were born at the expected Mendelian ratio, but fail to feed normally, exhibit gross bowel distension due to small bowel obstruction and die within 2 days post partum. Some, but not all, of the animals also have reduced pancreas size. pancreata had almost no expression of the islet hormones genes, except for PP (polypeptide-producing). SEQUENCE SIMILARITIES: Belongs to the RFX family.
Contains 1 H-T-H motif winged-type DNA-binding domain.
SEQUENCE CAUTION: The sequence AAI32105.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAI32295.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Immunohistochemistry in mouse embryo pancreas tissue.
Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Rfx6 in mouse embryo pancreas tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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