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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Porcine ELISA, WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MABN768 描述 Anti-PrP, clone DRM2-118 Antibody Alternate Names - CD230
- Major prion protein
- PrP
- PrP27-30
- PrP33-35C
背景信息 The protein named PrP is the cellular gene for the prion protein. The normal form appears to play a role in neuronal development and synaptic plasticity, iron transport and myelin sheath maintenance. PrP has both membrane associated and cytoplasmic/nuclear isoforms. The primary isoform is localized to the cellular membrane in lipid anchor clusters, as well as in the Golgi. Isoform 2 is cytoplasmic that becomes localized to the nucleus. Prp is normally a monomeric protein but a disease associated mutated form will form protease resistant oligomers often with another protein beta amyloid. The mutated form is found in high quantity in the brain of humans and animals infected with the degenerative neurological diseases such as Kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), Scrapie, and Bovine Spongiform Encephalopathy (BSE) and Transmissible Mink Encephalopathy. 产品信息 格式 Purified 演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-PrP, clone DRM2-118 | MABN768 is an antibody against PrP for use in Western Blotting, Immunohistochemistry, ELISA. 主要应用 - ELISA
- Western Blotting
- Immunohistochemistry
应用说明 Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PrP in 10 µg of rat brain tissue lysate.
ELISA Analysis: A representative lot detected PrP in recombinant PrP proteins from multiple species by ELISA using DRM1-31, DRM1-60, and DRM2-118 (Stanker, L.H., et al. (2012). 31(5):314-24).
Immunohistochemistry Analysis: A representative lot detected PrP in hamster brain sections tissue with DRM1-31, DRM1-60, and DRM2-118 (Stanker, L.H., et al. (2012). 31(5):314-24).
Western Blotting Analysis: A representative lot detected PrP in normal brain extract from multiple spcecies using DRM2-118. DRM1-60, and DRM1-31 lysate (Stanker, L.H., et al. (2012). 31(5):314-24).生物信息 免疫原品种 Recombinant protein corresponding to syrian hamster PrP. 克隆 DRM2-118 浓缩 Please refer to lot specific datasheet. 宿主 Mouse 特异性 DRM1-60 showed strong binding to only one peptide (#29: NQVYYRPVDQYS) suggesting a linear epitope corresponding to SHaPrP(159–170), located in b2-a2 loop. This epitope assignment is consistent with the binding of DRM1-60 to PrPC to all species tested, except human and horse, which both harbor substitutions in this region. While antibodies with epitopes similar to DRM2-118 have been described,(36) DRM1-60 and DRM1-31 have unique epitopes not previously reported. DRM1-60 appears to bind a linear epitope localized to the b2-a2 loop, while DRM1-31 has a conformational epitope located on the exposed surface formed by the close proximity of the b2-a2- helix loop, the N-terminus of the a2 helix, and the C-terminus of the a3 helix. 同种型 IgG2bκ 品种反应性 Porcine Species Reactivity Note Mouse, Rat, Bovine, Sheep, Hamster, Porcine, Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - XP_005068717
基因符号 - PRP
- PRNP
纯化方法 Protein G purified UniProt编号 - P04273
产品使用声明 质量保证 Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PrP in 10 µg of mouse brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,07-068,Anti-Calcineurin/PP2B Aβ Antibody
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