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品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, B, Fe, Chp IHC, WB, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 ABC11 Replaces 06-499 描述 Anti-Bax (NT) Antibody Alternate Names - apoptosis regulator BAX
- BCL2-associated X protein
- Bcl-2-like protein 4
背景信息 BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. 产品信息 格式 Purified 控制 - HL60 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Bax (N-terminus) Antibody is an antibody against Bax (N-terminus) for use in WB, IP, IC, IH. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated BAX from 500 µg of HL60 cell lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected BAX in colorectal and ductal carcinoma tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BAX in MCF7 cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human BAX at and around the N-terminus. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes BAX. 品种反应性 CanineHumanBovineFelineChimpanzee Species Reactivity Note Demonstrated to react with human. Predicted to react with bovine, feline, rhesus monkey, chimpanzee, and canine based on 100% sequence homology. Rat (90%), Mouse (86%). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_620119
Entrez基因汇总 The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq]. 基因符号 - Bcl2-L-4
- BAX
- BCL2L4
纯化方法 Protein A purfied UniProt编号 - Q07815
UniProt汇总 FUNCTION: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor Bcl-2 or its adenovirus homolog E1B 19k protein.| Q07812 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.
SIZE: 41 amino acids; 4678 Da
SUBUNIT: Interacts with HN.
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family
DISEASE: Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.
SIMILARITY: Belongs to the Bcl-2 family. | Q07814 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.# Belongs to the Bcl-2 family. | P55269 ## Belongs to the Bcl-2 family. | Q07812 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family (By similarity).# Belongs to the Bcl-2 family.产品使用声明 质量保证 Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 0.125 µg/ml of this antibody detected BAX on 10 µg of HL60 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,14-573,Pim1 Protein, active, 10 µg
下一件merck millipore产品：merck millipore,默克密理博,MABC002,Mouse IgG1 Negative Control, clone Ci4
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