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merck millipore,默克密理博,AB1784P,Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain
产品名称：Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain
产品型号：AB1784P
Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB.
merck millipore,默克密理博,AB1784P,Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1784P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain 产品信息 格式 Affinity Purified 控制 - POSITIVE CONTROL: rat striatal membranes (western blot)
演示 Affinity purified immunoglobulin. Liquid in PBS with 0.2% BSA and 0.05% sodium azide. 应用 应用 Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue sections. D1R has been localized in various regions of the rat brain (Huang, 1992; O'Connel, 1995)
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O'Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O'Connel, 1995) that is present in relatively small amounts.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.
The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A nine amino acid peptide corresponding to the 4th extracellular domain of the rat D1A receptor. The immunogen is absolutely conserved in the mouse D1A receptor and shows 88% homology with human and 77% with porcine and bovine D1A receptors. The peptide shows no significant sequence similarity with other dopamine receptors (D1B, D2-D5). 表位 4th Extracellular Domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Dopamine D1A Receptor (D1R). The rat D1A receptor is a 446 amino acid, G-protein coupled transmembrane receptor protein (Monsma, 1990; Zhou, 1990; Zhou, 1992; O'Dowd, 1990). The immunogen sequence has 88% sequence similarity with human and 77% with porcine. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000794.3
Entrez基因汇总 This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. 基因符号 - D
- DRD1
- DRD1A
- DADR
UniProt编号 - P21728
UniProt汇总 FUNCTION: SwissProt: P21728 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 446 amino acids; 49293 Da
SUBUNIT: Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD1IP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 (By similarity).
TISSUE SPECIFICITY: Detected in caudate, nucleus accumbens and in the olfactory tubercle.
SIMILARITY: SwissProt: P21728 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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