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merck millipore,默克密理博,AB982,Anti-Vasoactive Intestinal Peptide Antibody
产品名称：Anti-Vasoactive Intestinal Peptide Antibody
产品型号：AB982
Anti-Vasoactive Intestinal Peptide Antibody is an antibody against Vasoactive Intestinal Peptide for use in IH(P).
merck millipore,默克密理博,AB982,Anti-Vasoactive Intestinal Peptide Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Fe, Ca, Gp, H, M, R IH(P) Rb Serum Polyclonal Antibody 描述 产品目录编号 AB982 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Vasoactive Intestinal Peptide Antibody Alternate Names - VIP
产品信息 格式 Serum 控制 - Small bowel tissue with plexus attached
演示 Liquid antiSerum containing 0.1% sodium azide. 应用 应用 Anti-Vasoactive Intestinal Peptide Antibody is an antibody against Vasoactive Intestinal Peptide for use in IH(P). 主要应用 - Immunohistochemistry (Paraffin)
应用说明 TISSUE EVALUATION:
Formalin or alcohol fixed and paraffin-embedded sections (7-8 microns thick) of small bowel tissue prepared from a number of species including human, rat, cat, dog and guinea pig. Cryostat sections of the above tissue can also be used in the staining procedures.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:500-1:1,000 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes. Optimal dilution must be determined by the end user.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37°C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diamino-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated through alcohol, cleared in xylene and mounted.
IMMUNOFLUORESCENT
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C. Optimal dilution must be determined by the end user.
3. After washing off the excess antiserum with PBS, the section is treated with fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37°C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of gastrin cells using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours at either 37*C or 4*C.生物信息 免疫原品种 Synthetic (18-28) porcine vasoactive intestinal peptide bound to keyhole limpet hemocyanin (KLH) with carbodiimide. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antiserum supplied has been evaluated for its ability to stain ganglion cells in both the superficial and deep plexus of the wall of the human small bowel. 品种反应性 FelineCanineGuinea PigHumanMouseRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_194435.1
- NM_003381.2
Entrez基因汇总 The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. 基因符号 - PHM27
- VIP
- MGC13587
纯化方法 Unpurified UniProt编号 - P01282
UniProt汇总 FUNCTION: SwissProt: P01282 # PHM and PHV also cause vasodilation.
SIZE: 170 amino acids; 19169 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01282 ## Belongs to the glucagon family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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