- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,71072,pET-43.1 Ek/LIC Vector Kit - Novagen
产品名称：pET-43.1 Ek/LIC Vector Kit - Novagen
产品型号：71072
Novagen's pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus-Tag.
merck millipore,默克密理博,71072,pET-43.1 Ek/LIC Vector Kit - Novagen
- 产品介绍
描述 概述 pET-43.1 Ek/LIC Vector Kit
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.The pET-43.1 vectors incorporate the Nus•Tag fusion partner and are also compatible with the trxB/gor mutant Origami, Origami 2, Origami B, Rosetta-gami, Rosetta-gami 2, and Rosetta-gami B strains, which facilitatedisulfide bond formation in the cytoplasm (Davis 1998). Disulfide-bonded proteins maybe obtained by employing the combination of a pET-43.1 or pET-44 vector and these trxB/gor host strains. Unfused proteins can be produced by using the Nde I cloning site.
Directional PCR cloning into the most powerful E. coli expression vectors
The pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the Nus•Tag fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB318VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
The various pET Ek/LIC Vectors are shown below. Configurations of the pCDF-2 and pRSF-2 Ek/LIC Vectors, which are designed for coexpression, are also shown. For additional information about the LIC method and Ek/LIC primer design, refer to the previous section, The LIC Method. Ek/LIC vectors for insect and mammalian expression are also available.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.产品目录编号 71072 品牌系列 Novagen®
产品信息 部件 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 ≤ -70°C
无需冷冻 No
上一件merck millipore产品：merck millipore,默克密理博,370677,Guanosine 3ʹ,5ʹ-cyclic Monophosphorothioate, 8-(4-Chlorophenylthio)-, Rp-Isomer, Triethylammonium Salt - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,582005,Anti-Telomerase (Ab-2) Rabbit pAb
相关产品
merck millipore,默克密理博,189484,Autocamtide-2 Related Inhibitory Peptide II - Calbiochem
merck millipore,默克密理博,AB982,Anti-Vasoactive Intestinal Peptide Antibody
merck millipore,默克密理博,MAB858-2,Anti-RSV Antibody, glycoprotein, all type A, B strains, clone 131-2G
merck millipore,默克密理博,612151,Tissue Factor, Apo-, Human, Recombinant, S. frugiperda
merck millipore,默克密理博,582005,Anti-Telomerase (Ab-2) Rabbit pAb
merck millipore,默克密理博,573125,STAT3 Inhibitor IX, Cpd188 - CAS 823828-18-8 - Calbiochem
merck millipore,默克密理博,341599,FGF-10, Human, Recombinant
merck millipore,默克密理博,14-758M,Ret (V804L) Protein, active, 250 µg
