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品种反应性 主要应用 宿主 格式 抗体类型 Sh, B, H, M, R, Rb WB Sh Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB9740 Replaces 04-1089 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-14-3-3 Antibody, phospho Serine185 背景信息 Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.
The 14-3-3 phospho Serine185 antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.产品信息 格式 Affinity Purified 演示 Liquid in 10 mM Tris-HCl buffer, pH 7.5 with 150 mM NaCl, 100 ug/mL BSA, 50% glycerol and 0.05% sodium azide. 应用 应用 Detect 14-3-3 using this Anti-14-3-3 Antibody, phospho Serine185 validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1:150-1:300 using ECL.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic phospho peptide. 宿主 Sheep 特异性 14-3-3 phospho Serine185. By Western blot the antibody recognizes the ~30 kDa protein. Specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue. 品种反应性 SheepBovineHumanMouseRatRabbit 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006761.3
Entrez基因汇总 This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 基因符号 - FLJ45465
- YWHAE
- KCIP-1
- MDS
- 14-3-3E
- MDCR
修改 - Phosphorylation
纯化方法 Immunoaffinity purified UniProt编号 - P62258
UniProt汇总 FUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
SIZE: 255 amino acids; 29174 Da
SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,411543,Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate
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