merck millipore,默克密理愽
  • merck millipore,默克密理博,17-359,B-Raf Kinase Assay Kit, Chemiluminescence Detection

  • merck millipore,默克密理博,17-359,B-Raf Kinase Assay Kit, Chemiluminescence Detection

    产品名称：B-Raf Kinase Assay Kit, Chemiluminescence Detection
    产品型号：17-359
    Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user.

    merck millipore,默克密理博,17-359,B-Raf Kinase Assay Kit, Chemiluminescence Detection

  • 产品介绍
  • merck millipore,默克密理博,17-359,B-Raf Kinase Assay Kit, Chemiluminescence Detection

    重要规格表

    主要应用 可用的分析物 检测方法
    WBB-RafChemiluminescence
    描述
    产品目录编号 17-359
    品牌系列 Upstate
    商名
    • Upstate
    描述 B-Raf Kinase Assay Kit, Chemiluminescence Detection
    概述 Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user. For assay of B-Raf activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-B-Raf antibody (07-453).
    产品信息
    部件
    • Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Cat.# 07-461)
    • B-Raf (δ1-415), active (Cat.# 14-530)
    • MEK1, unactive (Cat.# 14-420)
    • Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
    • Magnesium/ATP Cocktail (Cat.# 20-113)
    检测方法 Chemiluminescence
    应用
    应用 Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user.
    主要应用
    • Western Blotting
    生物信息
    可用的分析物
    • B-Raf
    Entrez基因编号
    • NM_004333.3
    基因符号
    • RAFB1
    • BRAF
    • B-raf-1
    • BRAF1
    • MGC126806
    • B-Raf
    • p94
    • MGC138284
    UniProt编号
    • P15056
    UniProt汇总 FUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
    COFACTOR: Binds 2 zinc ions per subunit (By similarity).
    SIZE: 766 amino acids; 84437 Da
    SUBUNIT: Interacts with RIT1 (By similarity).
    SUBCELLULAR LOCATION: Cytoplasm.
    TISSUE SPECIFICITY: Brain and testis.
    DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
    SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
    产品使用声明
    质量保证 phosphorylation of MEK1
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 kit
    包装 Kit capacity: 50 assays

    merck millipore,默克密理博,17-359,B-Raf Kinase Assay Kit, Chemiluminescence Detection

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