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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Sh, H IH(P) Rb Serum Polyclonal Antibody 描述 产品目录编号 AB939 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Pancreatic Polypeptide Antibody Alternate Names - Pancreatic Hormone
产品信息 格式 Serum 演示 Liquid rabbit antiserum containing 0.1% sodium azide. 应用 应用 This Anti-Pancreatic Polypeptide Antibody is validated for use in IH(P) for the detection of Pancreatic Polypeptide. 主要应用 - Immunohistochemistry (Paraffin)
应用说明 TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (6 microns thick) of pancreatic tissue were obtained from a number of species including man and sheep. Cryostat sections of the above tissues can also be used in the staining procedures.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:500-1:3,000 (in PBS) is allowed to react with the tissue at 37°C for 30 min.
3. Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 min at 37ºC. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 min at 37°C.
3. After washing off the excess antiserum with PBS, the section is treated with fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 min at 37°C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of PPP cells using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hours at either 37°C or 2-8°C.生物信息 免疫原品种 Synthetic peptide corresponding to human pancreatic polypeptide (APLEPVYPGDNATPEQMAQYA ADLRRYINMLTRPRY) conjugated to to keyhole limpet hemocyanin (KLH) with carbodiimide. 宿主 Rabbit 特异性 The antiserum has been evaluated for its ability to stain cells in the pancreas containing pancreatic polypeptide-like (PPP) immunoreactivity using both the immunoperoxidase and immunofluorescence procedures. 品种反应性 SheepHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002722.3
Entrez基因汇总 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. 基因符号 - PP
- PPY
- PNP
UniProt编号 - P01298
UniProt汇总 FUNCTION: SwissProt: P01298 # The physiological role for the icosapeptide has not yet been elucidated.
SIZE: 95 amino acids; 10445 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01298 ## Belongs to the NPY family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20ºC in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µL
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